Suomi J, Wiedmer S K, Jussila M, Riekkola M L
Department of Chemistry, University of Helsinki, Finland.
Electrophoresis. 2001 Aug;22(12):2580-7. doi: 10.1002/1522-2683(200107)22:12<2580::AID-ELPS2580>3.0.CO;2-7.
A fast and easy method was sought for determination of the iridoid glycosides catalpol, ketologanin, verbenalin, loganin, 8-epi-loganic acid, geniposidic acid and 10-cinnamoyl catalpol in plant samples. The method involved micellar electrokinetic capillary chromatography (MEKC) coupled on-line to mass spectrometry. The partial filling technique and electrospray ionization were used. Seven iridoid glycosides could be separated with use of MEKC under basic conditions. However, 8-epi-loganic acid and geniposidic acid could not be detected simultaneously with the five neutral iridoid glycosides by mass spectrometry. Therefore, only the neutral iridoid glycosides were screened from plant samples. Catalpol, verbenalin, loganin and possibly 10-cinnamoyl catalpol were found in an examination of seven plant species in the genera Plantago, Veronica, Melampyrum, Succisa, and Valeriana. Aucubin, which was not included in the sample mixture used in method development because of overlapping with catalpol in MEKC, was also detected. The limits of detection for the iridoid glycosides, both at the UV and at the mass spectrometer, are given.
人们寻求一种快速简便的方法来测定植物样品中的环烯醚萜苷类成分，如梓醇、酮基龙胆酸、马鞭草苷、龙胆酸、8-表龙胆酸、栀子苷酸和10-肉桂酰梓醇。该方法采用胶束电动毛细管色谱法（MEKC）与质谱联用，使用了部分填充技术和电喷雾电离。在碱性条件下，利用MEKC可分离出七种环烯醚萜苷。然而，通过质谱法无法同时检测到8-表龙胆酸和栀子苷酸与五种中性环烯醚萜苷。因此，仅从植物样品中筛选出中性环烯醚萜苷。在对车前属、婆婆纳属、山萝花属、泽兰属和缬草属的七种植物进行检测时，发现了梓醇、马鞭草苷、龙胆酸以及可能的10-肉桂酰梓醇。由于在MEKC中与梓醇重叠，在方法开发中未包含在样品混合物中的桃叶珊瑚苷也被检测到。给出了环烯醚萜苷在紫外和质谱仪上的检测限。
