Pinna Antonio, Sechi Leonardo A, Zanetti Stefania, Usai Donatella, Carta Francesco
Institute of Ophthalmology, University of Sassari, Sassari, Italy.
Ophthalmology. 2004 Feb;111(2):348-51. doi: 10.1016/j.ophtha.2003.05.012.
We report the first case of bilateral contact lens-related Aeromonas caviae keratitis associated with A. caviae contamination of the contact lens case. The presence of virulence factors produced by Aeromonas species was also investigated.
Case report.
Conjunctival swabs and corneal scrapings from both eyes were inoculated for culture. The contact lens case was also cultured. The isolate was analyzed for the presence of virulence properties, such as gelatinase and protease production. The presence of virulence genes, such as the cytolytic enterotoxin (AHCYTOEN) and type IV Aeromonas pilus (tap) genes, was investigated using polymerase chain reaction. The susceptibility of A. caviae to 6 commercial contact lens disinfecting solutions was tested.
Culture results, protease activity, and gelatinase production were analyzed. Polymerase chain reaction amplification products were visualized in ethidium bromide-stained agarose gel. Bacterial growth after exposure to contact lens disinfecting solutions was assessed.
Aeromonas caviae was grown bilaterally from the conjunctiva, cornea, and contact lens case. The organism showed protease and gelatinase production. Polymerase chain reaction amplification revealed that the A. caviae strain contained the AHCYTOEN and tap virulence genes. Incubation for the minimum recommended time with all tested disinfecting solutions was effective in killing A. caviae.
Aeromonas caviae should be considered a possible etiologic agent of contact lens-associated keratitis. The presence of virulence factors may be important in determining corneal infection. Commercial contact lens disinfecting solutions, along with proper lens case hygiene, may be effective in preventing A. caviae keratitis in soft contact lens wearers.
我们报告首例与接触镜盒被豚鼠气单胞菌污染相关的双侧接触镜相关性豚鼠气单胞菌角膜炎病例。同时还研究了豚鼠气单胞菌产生的毒力因子。
病例报告。
对双眼的结膜拭子和角膜刮片进行接种培养。接触镜盒也进行培养。对分离菌株分析其毒力特性，如明胶酶和蛋白酶的产生情况。采用聚合酶链反应研究细胞溶解性肠毒素（AHCYTOEN）和IV型气单胞菌菌毛（tap）基因等毒力基因的存在情况。测试豚鼠气单胞菌对6种市售接触镜消毒溶液的敏感性。
分析培养结果、蛋白酶活性和明胶酶产生情况。聚合酶链反应扩增产物在溴化乙锭染色的琼脂糖凝胶中进行可视化观察。评估接触镜消毒溶液作用后细菌的生长情况。
豚鼠气单胞菌在双侧结膜、角膜和接触镜盒中均生长。该菌显示出蛋白酶和明胶酶的产生。聚合酶链反应扩增显示豚鼠气单胞菌菌株含有AHCYTOEN和tap毒力基因。所有测试的消毒溶液在推荐的最短时间孵育下均能有效杀灭豚鼠气单胞菌。
豚鼠气单胞菌应被视为接触镜相关性角膜炎的可能病原体。毒力因子的存在可能对确定角膜感染很重要。市售接触镜消毒溶液以及正确的镜盒卫生措施可能对预防软性接触镜佩戴者发生豚鼠气单胞菌角膜炎有效。
