Schiraldi Chiara, D'Agostino Antonella, Oliva Adriana, Flamma Floriana, De Rosa Alfredo, Apicella Antonio, Aversa Raffaella, De Rosa Mario
Section of Biotechnology and Molecular Biology, Department of Experimental Medicine, Second University of Naples, via De Crecchio 7, 80138 Naples, Italy.
Biomaterials. 2004 Aug;25(17):3645-53. doi: 10.1016/j.biomaterials.2003.10.059.
A novel hydrogel based on 2-hydroxyethylmethacrylate and fumed silica nanoparticles is presented. The filler was mixed at increasing amount (3-40% w/w) to the organic monomer, before accomplish thermal polymerization. The hybrid composite materials obtained were characterized as far as concern the physical-chemical stability and sorption behaviour in water and water solutions. The novel hybrid hydrogels were compared to poly(hydroxyethylmethacrylate) (pHEMA) on cytocompatibility and ability to elicit cell adhesion and proliferation. These in vitro assays showed that the first ones were supporting cell growth better then pHEMA, moreover experiments on murine fibroblasts showed improved adhesion and proliferation with the increase of the nanomeric filler content. For a more physiological response, the in vitro tests should match biomaterials with cell populations typical of the implant site. Therefore, in view of future applications of these composites as scaffolds for bone engineering, in a successive step of our research we selected primary cultures of human osteoblasts (OB) as the most appropriate models to study the in vitro performance of these materials. The preliminary results obtained confirmed the remarkable improvement of OB adhesion properties of the new hybrids with respect to pure pHEMA.
本文介绍了一种基于甲基丙烯酸2-羟乙酯和气相二氧化硅纳米颗粒的新型水凝胶。在进行热聚合之前，将填料以递增的量（3-40% w/w）与有机单体混合。对所得的杂化复合材料在水和水溶液中的物理化学稳定性及吸附行为进行了表征。将新型杂化水凝胶与聚（甲基丙烯酸2-羟乙酯）（pHEMA）在细胞相容性以及引发细胞黏附和增殖的能力方面进行了比较。这些体外试验表明，前者比pHEMA更能支持细胞生长，此外，对小鼠成纤维细胞的实验表明，随着纳米填料含量的增加，细胞的黏附和增殖有所改善。为了获得更符合生理的反应，体外试验应使生物材料与植入部位典型的细胞群体相匹配。因此，鉴于这些复合材料未来作为骨工程支架的应用，在我们研究的后续步骤中，我们选择人成骨细胞（OB）的原代培养物作为研究这些材料体外性能的最合适模型。所获得的初步结果证实，与纯pHEMA相比，新型杂化材料的OB黏附性能有显著改善。
