Pertz Olivier, Hahn Klaus M
The Scripps Research Institute, Department of Cell Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
J Cell Sci. 2004 Mar 15;117(Pt 8):1313-8. doi: 10.1242/jcs.01117.
Rho family GTPases are molecular switches that couple changes in the extracellular environment to intracellular signal transduction pathways. Their ability to regulate behaviors such as cell motility suggests very tight kinetic and spatial control of their activity, which is missed in most biochemical assays. Fluorescent probes that non-invasively report the changing subcellular location of Rho GTPase activity in single living cells are now allowing us to examine spatio-temporal regulation of the activity of these proteins, and are providing new biological insights. Several strategies can be used to construct such probes, and there are advantages and disadvantages associated with the diverse probe designs.
Rho家族GTP酶是分子开关，可将细胞外环境的变化与细胞内信号转导途径联系起来。它们调节细胞运动等行为的能力表明其活性受到非常严格的动力学和空间控制，而这在大多数生化分析中都被忽略了。能够无创报告单个活细胞中Rho GTP酶活性亚细胞定位变化的荧光探针，现在使我们能够研究这些蛋白质活性的时空调节，并提供新的生物学见解。可以使用几种策略来构建此类探针，并且不同的探针设计各有优缺点。
