Takahashi K, Oharaseki T, Wakayama M, Yokouchi Y, Naoe S, Murata H
Department of Pathology, Ohashi Hospital, Toho University School of Medicine, 2-17-6, Ohashi, Meguro, Tokyo, 153-8515, Japan.
Inflamm Res. 2004 Feb;53(2):72-7. doi: 10.1007/s00011-003-1225-1. Epub 2004 Jan 26.
We examined the histopathological features of systemic vasculitis caused in mice by injection of a Candida albicans ( C. albicans) extract and investigated the principal genetic roles in the development of vasculitis.
C. albicans extract was injected intraperitoneally for five consecutive days in the 1st and 5th weeks to CD-1, C57BL/6N, C3H/HeN, BALB/cAnN, DBA/2N and CBA/JN mice. At week 8, mice were killed, and histological examination was performed by light microscopy.
Arteritis had developed in 66% of CD-1 mice. The extramural coronary arteries and aortic root close to the orifice of coronary arteries were most frequently involved. Histologically, the characteristic feature of the arteritis was proliferative and granulomatous inflammation accompanied by numerous macrophages, lymphocytes, plasma cells and neutrophils. Fibrocellular intimal thickening with destruction of the internal elastic lamina and media was also observed. Five mouse strains after injection of C. albicans extract were clearly classified into a resistant group (CBA/JN, DBA/2N and BALB/cAnN mice) and a sensitive group (C3H/HeN and C57BL/6N mice). The inbred mouse strains which showed the same histocompatibility-2 (H-2) haplotype exhibited a different susceptibility to development of vasculitis.
This arteritis murine model shows unique histological features that have not been observed in other animal vasculitis models and it most closely resembles Kawasaki disease in humans. The genetic control of susceptibility to induction of vasculitis by the C. albicans extract is dependent to the mouse strains, but is not linked to the H-2 loci.
我们研究了通过注射白色念珠菌提取物在小鼠中引发的系统性血管炎的组织病理学特征，并探讨了血管炎发展过程中的主要基因作用。
在第1周和第5周连续5天向CD-1、C57BL/6N、C3H/HeN、BALB/cAnN、DBA/2N和CBA/JN小鼠腹腔注射白色念珠菌提取物。在第8周，处死小鼠，并通过光学显微镜进行组织学检查。
66%的CD-1小鼠发生了动脉炎。最常受累的是壁外冠状动脉和靠近冠状动脉口的主动脉根部。组织学上，动脉炎的特征性表现为增殖性和肉芽肿性炎症，伴有大量巨噬细胞、淋巴细胞、浆细胞和中性粒细胞。还观察到纤维细胞内膜增厚伴内弹性膜和中膜破坏。注射白色念珠菌提取物后的5种小鼠品系可明显分为抗性组（CBA/JN、DBA/2N和BALB/cAnN小鼠）和敏感组（C3H/HeN和C57BL/6N小鼠）。具有相同组织相容性-2（H-2）单倍型的近交小鼠品系对血管炎发展表现出不同的易感性。
这种动脉炎小鼠模型显示出独特的组织学特征，这在其他动物血管炎模型中未观察到，并且与人类川崎病最为相似。白色念珠菌提取物诱导血管炎易感性的遗传控制取决于小鼠品系，但与H-2基因座无关。
