D-和L-环己烯核酸（CeNA）的碱基配对特性。

Base pairing properties of D- and L-cyclohexene nucleic acids (CeNA).

作者信息

Gu Ping, Schepers Guy, Rozenski Jef, Van Aerschot Arthur, Herdewijn Piet

机构信息

Laboratory for Medicinal Chemistry, Rega Institute for Medical Research, B-3000 Leuven, Belgium.

出版信息

Oligonucleotides. 2003;13(6):479-89. doi: 10.1089/154545703322860799.

DOI:10.1089/154545703322860799

PMID:15025914

Abstract

Cyclohexene nucleic acids (CeNA) with a D-like configuration form very stable self-complementary duplexes and stable duplexes with RNA. An increased duplex stability with Delta T(m)/mod of +1.2 degrees C is observed. The duplex with DNA is less stable. Excellent mismatch discrimination has been observed as well for the duplex with DNA as for the duplex with RNA. The results obtained with mixed CeNA sequences warrant antisense studies with CeNA. The CeNAs of opposite chirality constitute a self-pairing system on their own, resembling L-RNA sequences.

摘要

具有D型构型的环己烯核酸（CeNA）能形成非常稳定的自身互补双链体以及与RNA的稳定双链体。观察到双链体稳定性增加，ΔT(m)/mod为 +1.2℃。与DNA形成的双链体稳定性较差。对于与DNA形成的双链体以及与RNA形成的双链体，均观察到了出色的错配识别能力。用混合CeNA序列获得的结果为CeNA的反义研究提供了依据。具有相反手性的CeNA自身构成一个自配对系统，类似于L-RNA序列。