Rong Ju, Xu Li-Yan, Cai Wei-Jia, Xiong Xing-Dong, Li Jin-Tao, Fang Wang-Kai, Shen Zhong-Ying, Li En-Min
Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou, Guangdong, 515041 PR China.
Ai Zheng. 2004 Mar;23(3):243-8.
BACKGROUND & OBJECTIVE: Fascin 1 is the 55kDa F-actin- binding cytoskeleton protein. Fascin 1 gene was cloned from a human teratocarcinoma. Up to now, the carcinogenesis mechanism of esophageal squamous cell carcinoma is unclear. The study was designed to identify the differentially expressed proteins and mRNAs between the human immortalized esophageal epithelial cell line (SHEE) transfected by human papillomavirus type 18 E6E7 and the malignant transformation cell line (SHEEmt), which is derivated from SHEE, and to further understand the carcinogenesis mechanisms of esophageal squamous cell carcinoma.
Cellular proteins were separated by two-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS). The mRNA of fascin 1 gene was assayed by reverse transcription polymerase chain reaction (RT-PCR) and its product was analyzed by sequencing assay.
The results manifested 9 genes expressed differently in the progress of malignant transformation of SHEE to SHEEmt, fascin 1 protein increased about 3.64 times and its mRNA increased about 16.17 times.
The upregulated expression of fascin 1 gene may be correlated with the malignant transformation of SHEE to SHEEmt.
Fascin 1是一种55kDa的F-肌动蛋白结合细胞骨架蛋白。Fascin 1基因是从人畸胎瘤中克隆出来的。目前，食管鳞状细胞癌的致癌机制尚不清楚。本研究旨在鉴定人乳头瘤病毒18型E6E7转染的人永生化食管上皮细胞系（SHEE）与其衍生的恶性转化细胞系（SHEEmt）之间差异表达的蛋白质和mRNA，以进一步了解食管鳞状细胞癌的致癌机制。
采用双向电泳分离细胞蛋白，用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）鉴定差异表达的蛋白质。采用逆转录聚合酶链反应（RT-PCR）检测Fascin 1基因的mRNA，并通过测序分析其产物。
结果显示，在SHEE向SHEEmt恶性转化过程中有9个基因表达差异，Fascin 1蛋白增加约3.64倍，其mRNA增加约16.17倍。
Fascin 1基因的上调表达可能与SHEE向SHEEmt的恶性转化有关。
