Jin Pei, Fu Glenn K, Wilson Amy D, Yang Junming, Chien David, Hawkins Phillip R, Au-Young Janice, Stuve Laura L
Incyte Corporation, 3160 Porter Drive, Palo Alto, CA 94304, USA.
Genomics. 2004 Apr;83(4):566-71. doi: 10.1016/j.ygeno.2003.09.023.
Alternative splicing of pre-mRNAs is an important mechanism for the generation of vertebrate protein diversity. Unfortunately, the contribution of alternative splicing to protein diversity is currently not well understood because many full-length mRNA sequences have yet to be identified. In this report, we describe the use of RT-PCR to identify and clone 279 novel alternatively spliced mRNAs from 114 well-known drug target genes. Our findings demonstrate the existence of many novel alternatively spliced mRNA transcripts and suggest that many more genes undergo functionally significant alternative splicing than previously thought.
前体mRNA的可变剪接是产生脊椎动物蛋白质多样性的重要机制。遗憾的是，由于许多全长mRNA序列尚未被鉴定出来，目前可变剪接对蛋白质多样性的贡献还没有得到很好的理解。在本报告中，我们描述了使用逆转录聚合酶链反应（RT-PCR）从114个知名药物靶基因中鉴定和克隆279个新的可变剪接mRNA。我们的研究结果证明了许多新的可变剪接mRNA转录本的存在，并表明经历功能上重要的可变剪接的基因比以前认为的要多得多。
