McGillivery D J, Yong W K, Adler B, Riffkin G G
Department of Agriculture, Regional Veterinary Laboratory, Hamilton, Victoria, Australia.
Vaccine. 1992;10(9):607-13. doi: 10.1016/0264-410x(92)90441-l.
The 31 kDa antigen of third-stage (L3) Ostertagia circumcincta larvae was evaluated as a potential prophylactic antigen by an analysis of the protective, humoral and cell-mediated responses of lambs immunized with this antigen. Six lambs were immunized by subcutaneous injection with a total of 400 micrograms of the purified 31 kDa antigen in 250 micrograms ml-1 Quil A adjuvant. Five sheep given identical injections but without the 31 kDa antigen were used as controls. All animals were challenged with 4.2 x 10(4) infective L3 O. circumcincta larvae 1 week after the last booster injection. The protection afforded by the 31 kDa antigen was demonstrated by a significant reduction in faecal egg counts (p less than 0.05) and total worm counts (p less than 0.005) in vaccinated animals. Elevated ELISA antibody levels specific to the 31 kDa antigen were detected in the sera of vaccinated animals as early as 3 weeks after immunization. Specific antibodies were further demonstrated by Western blot 4 days after the first booster immunization at 3 weeks. In control animals no antibodies to the 31 kDa antigen were detected in Western blots throughout the course of the experiment. Immunized lambs had significantly higher in vitro lymphocyte stimulation indices (p less than 0.05) than control animals. In haematoxylin and eosin stained sections taken at necropsy from vaccinated sheep, compared with control lambs, there were more larvae present in the crypts of the abomasal epithelium and these larvae were surrounded by lymphocytes and eosinophils. In vaccinated lambs a marked infiltration of lymphocytes in the lamina propria and oedema in the submucosa were also observed.(ABSTRACT TRUNCATED AT 250 WORDS)
通过分析用该抗原免疫的羔羊的保护性、体液和细胞介导反应，对环形奥斯特他线虫第三期（L3）幼虫的31 kDa抗原作为潜在的预防性抗原进行了评估。六只羔羊通过皮下注射，在250微克/毫升的Quil A佐剂中总共注射400微克纯化的31 kDa抗原进行免疫。另外五只绵羊接受相同注射但不含31 kDa抗原，用作对照。在最后一次加强注射1周后，所有动物均用4.2×10⁴条感染性L3环形奥斯特他线虫幼虫进行攻击。接种动物粪便虫卵计数（p<0.05）和总虫数（p<0.005）显著减少，证明了31 kDa抗原提供的保护作用。早在免疫后3周，就在接种动物的血清中检测到针对31 kDa抗原的ELISA抗体水平升高。首次加强免疫3周后4天，通过蛋白质印迹进一步证实了特异性抗体。在整个实验过程中，对照动物的蛋白质印迹中未检测到针对31 kDa抗原的抗体。免疫羔羊的体外淋巴细胞刺激指数显著高于对照动物（p<0.05）。在接种绵羊尸检时采集的苏木精和伊红染色切片中，与对照羔羊相比，皱胃上皮隐窝中有更多幼虫，这些幼虫被淋巴细胞和嗜酸性粒细胞包围。在接种羔羊中还观察到固有层淋巴细胞明显浸润和黏膜下层水肿。（摘要截断于250字）
