一种微生物传感器在生乳样品中短链脂肪酸测定中的应用。

Application of a microbial sensor for determination of short-chain fatty acids in raw milk samples.

作者信息

Ukeda H, Wagner G, Weis G, Miller M, Klostermeyer H, Schmid R D

机构信息

GBF-Gesellschaft für Biotechnische Forschung, Department of Enzymtechnology, Braunschweig, Federal Republic of Germany.

出版信息

Z Lebensm Unters Forsch. 1992 Jul;195(1):1-2. doi: 10.1007/BF01197829.

DOI:10.1007/BF01197829

PMID:1502853

Abstract

A microbial sensor system, based on the use of immobilized Arthrobacter nicotiana and an oxygen electrode, was applied to determine free short-chain fatty acids in raw milk samples and the result was compared with gas chromatography (GC) and a titrimetric method. The sensor response was linearly related to the concentration of short-chain fatty acids obtained by GC (n = 10, r = 0.92) and to the total concentration of free fatty acids obtained by titrimetric measurement (n = 10, r = 0.78). This result suggests that the present microbial sensor can selectively determine free short-chain fatty acids in raw milk samples and may be useful as a very fast detection method of rancidity in milk.

摘要

一种基于固定化烟草节杆菌和氧电极的微生物传感器系统被用于测定生乳样品中的游离短链脂肪酸，并将结果与气相色谱法（GC）和滴定法进行比较。传感器响应与通过GC获得的短链脂肪酸浓度呈线性相关（n = 10，r = 0.92），与通过滴定测量获得的游离脂肪酸总浓度呈线性相关（n = 10，r = 0.78）。该结果表明，目前的微生物传感器可以选择性地测定生乳样品中的游离短链脂肪酸，并且可能作为一种非常快速的牛奶酸败检测方法。