炭疽芽孢杆菌和蜡样芽孢杆菌的PcrA解旋酶能够支持金黄色葡萄球菌质粒pT181的DNA解旋及体外滚环复制。
Bacillus anthracis and Bacillus cereus PcrA helicases can support DNA unwinding and in vitro rolling-circle replication of plasmid pT181 of Staphylococcus aureus.
作者信息
Anand Syam P, Mitra Poulami, Naqvi Asma, Khan Saleem A
机构信息
Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA.
出版信息
J Bacteriol. 2004 Apr;186(7):2195-9. doi: 10.1128/JB.186.7.2195-2199.2004.
Abstract
Replication of rolling-circle replicating (RCR) plasmids in gram-positive bacteria requires the unwinding of initiator protein-nicked plasmid DNA by the PcrA helicase. In this report, we demonstrate that heterologous PcrA helicases from Bacillus anthracis and Bacillus cereus are capable of unwinding Staphylococcus aureus plasmid pT181 from the initiator-generated nick and promoting in vitro replication of the plasmid. These helicases also physically interact with the RepC initiator protein of pT181. The ability of PcrA helicases to unwind noncognate RCR plasmids may contribute to the broad-host-range replication and dissemination of RCR plasmids in gram-positive bacteria.
摘要
滚环复制（RCR）质粒在革兰氏阳性菌中的复制需要PcrA解旋酶解开引发蛋白切口的质粒DNA。在本报告中，我们证明来自炭疽芽孢杆菌和蜡样芽孢杆菌的异源PcrA解旋酶能够从引发剂产生的切口解开金黄色葡萄球菌质粒pT181，并促进该质粒的体外复制。这些解旋酶还与pT181的RepC引发蛋白发生物理相互作用。PcrA解旋酶解开非同源RCR质粒的能力可能有助于RCR质粒在革兰氏阳性菌中的广泛宿主范围复制和传播。
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