Galán Beatriz, García José L, Prieto María A
Department of Molecular Microbiology, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
J Bacteriol. 2004 Apr;186(7):2215-20. doi: 10.1128/JB.186.7.2215-2220.2004.
The pac gene, encoding the penicillin G acylase from Escherichia coli W, is regulated by the PaaX repressor of the phenylacetate catabolic pathway. pac expression depends on the synthesis of phenylacetyl-coenzyme A. PaaX and the cyclic AMP receptor protein (CRP) bind in vitro to the Ppac promoter region. A palindromic sequence proposed as the PaaX operator is located upstream of the -35 box overlapping a CRP binding site, an unusual position that suggests a novel regulatory mechanism.
编码来自大肠杆菌W的青霉素G酰化酶的pac基因，受苯乙酸分解代谢途径的PaaX阻遏物调控。pac的表达取决于苯乙酰辅酶A的合成。PaaX和环腺苷酸受体蛋白（CRP）在体外与Ppac启动子区域结合。一个被认为是PaaX操纵子的回文序列位于-35框上游，与一个CRP结合位点重叠，这一不同寻常的位置提示了一种新的调控机制。
