Gibson Bradford W
Buck Institute for Age Research, Novato, CA 94945, USA.
Sci Aging Knowledge Environ. 2004 Mar 17;2004(11):pe12. doi: 10.1126/sageke.2004.11.pe12.
To understand how oxidative stress contributes to aging and age-related diseases and to better evaluate the therapeutic effect of antioxidant drugs, it would be highly desirable to have a comprehensive and detailed readout of the types of oxidative damage that occur to proteins at a global or proteome level. In this Perspective, I examine how proteomics, defined here as the science of examining all proteins in an organelle, cell, or tissue in the context of biological phenomena, can be used to provide molecular details of mitochondrial protein oxidative damage. Specifically, I discuss approaches that combine knowledge of the mitochondrial proteome with newer mass spectrometry-based techniques that are capable of identifying proteins and sites of oxidative modification in a high-throughput manner.
为了解氧化应激如何导致衰老及与年龄相关的疾病，并更好地评估抗氧化药物的治疗效果，非常需要在整体或蛋白质组水平上全面、详细地读出蛋白质发生的氧化损伤类型。在这篇观点文章中，我探讨了蛋白质组学（这里定义为在生物学现象背景下研究细胞器、细胞或组织中所有蛋白质的科学）如何能够用于提供线粒体蛋白质氧化损伤的分子细节。具体而言，我讨论了将线粒体蛋白质组知识与基于质谱的更新技术相结合的方法，这些技术能够以高通量方式鉴定蛋白质及氧化修饰位点。
