Kommoss F, Bauknecht T, Birmelin G, Kohler M, Tesch H, Pfleiderer A
Department of Gynaecology, Albert-Ludwig University, Freiburg, Germany.
Acta Obstet Gynecol Scand Suppl. 1992;155:19-24. doi: 10.1111/j.1600-0412.1992.tb00003.x.
The varying tumor-biological behavior of ovarian carcinomas probably influences both their operability and response to chemotherapy, which are the most relevant prognostic factors. The phenotype of different ovarian carcinomas is obviously associated with an activation of the EGF/TGF-alpha signal pathway, including c-myc and c-jun expression. Analysis of EGF-R, TGF-alpha, c-myc and c-jun expression in 33 stage III/IV, and 2 stage I/II ovarian carcinomas with biochemical, molecular-chemical and immunohistochemical methods showed a correlation between the mRNA and protein levels of EGF-R and TGF-alpha for tumors with low or high expressing rates. However, the concentration of measurable free EGF-Rs seems to depend on the amount of TGF-alpha expression by the tumors. The EGF-R binding ligand TGF-alpha is produced by epithelial tumor cells; stromal cells are usually TGF-alpha-negative, as shown by immunohistochemistry. High expression rates of EGF-R. TGF-alpha and c-myc were detected in 6, 7, and 10 out of 35 ovarian carcinomas, respectively. C-jun mRNA was detected in 18/19 cases studied. Non-malignant tissues originating from myometrium or ovary expressed no (or only small amounts of) EGF-R or TGF-alpha mRNA, whereas a high c-myc expression was found in 1/7 normal myometria, and in 2/5 normal ovaries. There was no strong correlation between EGF-R/TGF-alpha and c-myc/c-jun expression.(ABSTRACT TRUNCATED AT 250 WORDS)
卵巢癌不同的肿瘤生物学行为可能会影响其可切除性和对化疗的反应，而这两者是最相关的预后因素。不同卵巢癌的表型显然与表皮生长因子/转化生长因子-α（EGF/TGF-α）信号通路的激活有关，包括c-myc和c-jun的表达。采用生化、分子化学和免疫组化方法对33例III/IV期和2例I/II期卵巢癌的EGF-R、TGF-α、c-myc和c-jun表达进行分析，结果显示，对于表达率低或高的肿瘤，EGF-R和TGF-α的mRNA水平与蛋白水平之间存在相关性。然而，可测量的游离EGF-Rs浓度似乎取决于肿瘤TGF-α的表达量。EGF-R结合配体TGF-α由上皮肿瘤细胞产生；免疫组化显示，基质细胞通常为TGF-α阴性。在35例卵巢癌中，分别有6例、7例和10例检测到EGF-R、TGF-α和c-myc的高表达率。在所研究的19例病例中有18例检测到c-jun mRNA。源自子宫肌层或卵巢的非恶性组织不表达（或仅少量表达）EGF-R或TGF-α mRNA，而在7例正常子宫肌层中有1例、5例正常卵巢中有2例发现c-myc高表达。EGF-R/TGF-α与c-myc/c-jun表达之间没有强相关性。（摘要截选至250字）
