Park Se-Keun, Pak Kyung-Ran, Choi Sung-Chan, Kim Yeong-Kwan
Department of Environmental Engineering, Kangwon National University, Chunchon, Korea.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2004;39(1):103-12. doi: 10.1081/ese-120027371.
Three different bioassays for analysis of biodegradable dissolved organic carbon (BDOC) were evaluated to identify which method is most applicable to analysis of drinking water. The determination of BDOC is primarily based on the differences between initial and final DOC levels during a certain incubation period using indigenous bacterial consortium as an inoculum. The assay procedures basically differ in the preparation method of inoculum. Inoculum was added in the form of suspended bacteria in one assay. In the other two assays, bacterial inoculum attached to either sand or inert media was used in a continuous reactor column. Standard solutions containing sodium acetate, sodium oxalate, or glucose at 1 mg C/L, and tap water were tested. The bioassay using bacteria attached to sand was shown to be superior to the two other methods for BDOC determination in terms of its incubation period, recovery, and reproducibility. Tap water samples, when analyzed by this assay, could not be guaranteed for biological stability due to their high BDOC concentrations (0.17 to 0.23 mg/L) that corresponded to 26-36% of the initial DOC level.
对三种用于分析可生物降解溶解性有机碳（BDOC）的不同生物测定法进行了评估，以确定哪种方法最适用于饮用水分析。BDOC的测定主要基于在特定培养期内使用本地细菌群落作为接种物时初始和最终DOC水平之间的差异。测定程序在接种物的制备方法上基本不同。在一种测定法中，接种物以悬浮细菌的形式添加。在另外两种测定法中，附着在沙子或惰性介质上的细菌接种物用于连续反应器柱中。测试了含有1 mg C/L乙酸钠、草酸钠或葡萄糖的标准溶液以及自来水。就培养期、回收率和重现性而言，使用附着在沙子上的细菌的生物测定法在BDOC测定方面优于其他两种方法。当通过该测定法分析自来水样品时，由于其高BDOC浓度（0.17至0.23 mg/L），相当于初始DOC水平的26-36%，因此无法保证其生物稳定性。
