Yu Peiqiang, McKinnon John J, Christensen Colleen R, Christensen David A
College of Agriculture, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan S7N 5A8, Canada.
J Agric Food Chem. 2004 Mar 24;52(6):1484-94. doi: 10.1021/jf035065a.
The objective of this study was to use synchrotron transmission FTIR microspectroscopy as a rapid, direct, and nondestructive analytical technique to reveal molecular microstructural-chemical features within tissue in grain barley. The results showed that synchrotron transmission FTIR microspectroscopy could provide spectral, chemical, and functional group characteristics of grain barley tissue at ultrahigh spatial resolutions. The spatially localized structural-chemical distributions of biological components (lignin, cellulose, protein, lipid, and carbohydrates) and biological component ratios could be imaged. Such information on molecular microstructural-chemical features within the tissue can be used for plant breeding programs for selecting superior varieties of barley for special purposes and for prediction of grain barley quality and nutritive value for humans and animals.
本研究的目的是使用同步辐射傅里叶变换红外显微光谱技术作为一种快速、直接且无损的分析技术，以揭示大麦籽粒组织内的分子微观结构化学特征。结果表明，同步辐射傅里叶变换红外显微光谱技术能够在超高空间分辨率下提供大麦籽粒组织的光谱、化学和官能团特征。生物成分（木质素、纤维素、蛋白质、脂质和碳水化合物）的空间定位结构化学分布以及生物成分比率可以成像。组织内分子微观结构化学特征的此类信息可用于植物育种计划，以选择用于特殊目的的优质大麦品种，并用于预测大麦籽粒对人类和动物的品质及营养价值。
