Rajesh Logasundaram, Pattari Sanjib Kumar, Garewal Gurjeewan, Dey Pranab, Srinivasan Radhika
Department of Pathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Anal Quant Cytol Histol. 2004 Feb;26(1):57-60.
To determine if image morphometry has any role in distinguishing blasts of acute lymphoblastic leukemia (ALL-L2) from those of acute myeloid leukemia (AML-M1) and (AML-M2).
Ten cases each of ALL-L2, AML-M1 and AML-M2 diagnosed according to the French-American-British criteria were studied. In all cases May-Grünwald-Giemsa-stained bone marrow aspiration smears were obtained. At least 100 blast cells from each case were subjected to analysis randomly with an image cytometer using Leica Quantimet 600 software (Cambridge, U.K.). The area, convex area, length, width, perimeter, convex perimeter, roundness, total optical density, average optical density and pixel grey value variance of the nuclei were measured by random selection of cells using a 40:1 objective (1 pixel = 0.446 micron).
Mann Whitney's nonparametric test showed that there was considerable overlap of morphometric variables between the 3 subtypes. Though statistical significance was found in "roundness" between blasts of AML-M1 and ALL-L2, power analyses (sample size of 100 blasts of each subtype) did not show sufficient power for this variable. However, between blasts of ALL-L2 and AML-M2, "average optical density" and "pixel grey value variance" were statistically significant with full power using power analyses.
Image morphometry may be helpful in differentiating blasts from lymphoid and myeloid leukemic subtypes.
确定图像形态测量法在区分急性淋巴细胞白血病（ALL-L2）的原始细胞与急性髓系白血病（AML-M1）和（AML-M2）的原始细胞方面是否有作用。
对根据法美英标准诊断的10例ALL-L2、10例AML-M1和10例AML-M2病例进行研究。所有病例均获取了经May-Grünwald-Giemsa染色的骨髓穿刺涂片。使用徕卡Quantimet 600软件（英国剑桥）的图像细胞仪对每个病例中至少100个原始细胞进行随机分析。通过使用40:1物镜随机选择细胞（1像素 = 0.446微米）来测量细胞核的面积、凸面积、长度、宽度、周长、凸周长、圆度、总光密度、平均光密度和像素灰度值方差。
Mann Whitney非参数检验表明，这3种亚型之间的形态测量变量存在相当大的重叠。虽然在AML-M1和ALL-L2的原始细胞之间的“圆度”方面发现了统计学意义，但功效分析（每个亚型100个原始细胞的样本量）并未显示该变量有足够的功效。然而，在ALL-L2和AML-M2的原始细胞之间，使用功效分析，“平均光密度”和“像素灰度值方差”具有统计学意义且功效充分。
图像形态测量法可能有助于区分淋巴系和髓系白血病亚型的原始细胞。
