McLuckey S A, Goeringer D E, Glish G L
Analytical Chemistry Division, Oak Ridge National Laboratory, Tennessee 37831-6365.
Anal Chem. 1992 Jul 1;64(13):1455-60. doi: 10.1021/ac00037a026.
Random noise applied to the end caps of a quadrupole ion trap is shown to be an effective means for the collisional activation of trapped ions independent of mass/charge ratio and number of ions. This technique is compared and contrasted with conventional single-frequency collisional activation for the molecular ion of N,N-dimethylaniline, protonated cocaine, the molecular anion of 2,4,6-trinitrotoluene, and doubly pronated neuromedin U-8. Collisional activation with noise tends to produce more extensive fragmentation than the conventional approach due to the fact that product ions are also kinetically excited in the noise experiment. The efficiency of the noise experiment in producing detectable product ions relative to the conventional approach ranges from being equivalent to being a factor of 3 less efficient. Furthermore, discrimination against low mass/charge product ions is apparent in the data from multiply charged biomolecules. Nevertheless, collisional activation with random noise provides a very simple means for overcoming problems associated with the dependence of single-frequency collisional activation on mass/charge ratio and the number of ions in the ion trap.
已证明，施加于四极杆离子阱端盖的随机噪声是使捕获离子发生碰撞活化的有效手段，与质荷比和离子数量无关。将该技术与N,N - 二甲基苯胺分子离子、质子化可卡因、2,4,6 - 三硝基甲苯分子阴离子以及双质子化神经降压素U - 8的传统单频碰撞活化进行了比较和对比。与传统方法相比，噪声碰撞活化往往会产生更广泛的碎片化，因为在噪声实验中产物离子也会被动力学激发。相对于传统方法，噪声实验产生可检测产物离子的效率范围从相当到低3倍。此外，在多电荷生物分子的数据中，对低质荷比产物离子的歧视很明显。然而，随机噪声碰撞活化为克服与单频碰撞活化对质荷比和离子阱中离子数量的依赖性相关的问题提供了一种非常简单的方法。
