金属离子与4-（2-噻唑基偶氮）间苯二酚在毛细管电泳中的毛细管络合作用
On-capillary complexation of metal ions with 4-(2-thiazolylazo)resorcinol in capillary electrophoresis.
作者信息
Wang Min, Lin Jin-Ming, Qu Feng, Shan Xiaoquan, Chen Zuliang
机构信息
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.
出版信息
J Chromatogr A. 2004 Mar 12;1029(1-2):249-54. doi: 10.1016/j.chroma.2003.12.011.
Co(II), Zn(II), Ni(II) and Fe(I) were successfully separated by capillary electrophoresis using pre-capillary and on-capillary complexation with 4-(2-thiazolylazo)resorcinol. The influences of some crucial parameters, including both pre- and on-capillary complexation procedure, were investigated. For on-capillary complexation, the complexing reaction was carried out inside the capillary by mixing the zones of ligand and sample during the electrophoretic migration. Compared with pre-capillary complexation, the method provided 30-fold reduction in detection limits for Co(II), 50-fold reduction for Zn(II), and 100-fold reduction for Ni(II) and Fe(II). It was used for the analysis of a pharmaceutical and tap water sample.
通过使用4-(2-噻唑基偶氮)间苯二酚进行预毛细管和毛细管内络合，成功地利用毛细管电泳分离了钴(II)、锌(II)、镍(II)和铁(I)。研究了一些关键参数的影响，包括预毛细管和毛细管内络合过程。对于毛细管内络合，络合反应在电泳迁移过程中通过混合配体区和样品区在毛细管内进行。与预毛细管络合相比，该方法使钴(II)的检测限降低了30倍，锌(II)降低了50倍，镍(II)和铁(II)降低了100倍。它被用于药物和自来水样品的分析。
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