Ohtori S, Isogai E, Hasue F, Ozaki T, Nakamura Y, Nakagawara A, Koseki H, Yuasa S, Hanaoka E, Shinbo J, Yamamoto T, Chiba H, Yamazaki M, Moriya H, Sakiyama S
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chuo, Chiba 260-8677, Japan.
Mol Cell Neurosci. 2004 Mar;25(3):504-14. doi: 10.1016/j.mcn.2003.12.002.
Differential screening-selected gene aberrative in neuroblastoma (Dan) protein is produced in small neurons of dorsal root ganglia. Thermal and mechanical allodynia and Fos expression in the spinal dorsal horn evoked by inflammation and neuropathic pain were investigated using Dan-deficient mice. Mice showed pain reactions induced by the introduction of complete Freund's adjuvant (CFA) into their hind paw (inflammatory pain model) and after sciatic nerve ligation (neuropathic pain model). In the inflammatory pain model, thermal and mechanical pain thresholds in Dan-deficient mice were significantly higher than those of wild-type mice. The number of Fos-immunoreactive cells in the dorsal horn during the inflammatory period was significantly less in Dan-deficient mice. However, in the neuropathic pain model, no differences in thermal hypersensitivity, mechanical allodynia, or the number of Fos-immunoreactive cells in the dorsal horn were observed between the mice. These data suggest that Dan may be a neuromodulator in inflammatory pain.
差异筛选选择的神经母细胞瘤异常基因（Dan）蛋白在背根神经节的小神经元中产生。使用Dan基因缺陷小鼠研究了炎症和神经性疼痛诱发的脊髓背角中的热和机械性异常性疼痛以及Fos表达。小鼠在将完全弗氏佐剂（CFA）注入其后爪后（炎症性疼痛模型）以及坐骨神经结扎后（神经性疼痛模型）表现出疼痛反应。在炎症性疼痛模型中，Dan基因缺陷小鼠的热和机械性疼痛阈值显著高于野生型小鼠。炎症期背角中Fos免疫反应性细胞的数量在Dan基因缺陷小鼠中显著减少。然而，在神经性疼痛模型中，小鼠之间在热超敏反应、机械性异常性疼痛或背角中Fos免疫反应性细胞数量方面未观察到差异。这些数据表明Dan可能是炎症性疼痛中的一种神经调节剂。
