通过竞争性逆转录聚合酶链反应对含有山梨酸或亚硝酸钠的培养基中肉毒梭菌E型毒素编码mRNA进行定量分析。
Quantification of toxin-encoding mRNA from Clostridium botulinum type E in media containing sorbic acid or sodium nitrite by competitive RT-PCR.
作者信息
Sharkey Freddie H, Markos Spiros I, Haylock Richard W
机构信息
School of Biological and Environmental Sciences, University of Ulster, Cromore Road, Coleraine, Co. Derry BT52 1SA, UK.
出版信息
FEMS Microbiol Lett. 2004 Mar 19;232(2):139-44. doi: 10.1016/S0378-1097(04)00043-6.
Competitive reverse transcription polymerase chain reaction (cRT-PCR) was used to quantify the toxin-encoding mRNA production of a Clostridium botulinum type E strain in media containing either sorbic acid or sodium nitrite. A 10-fold reduction in toxin mRNA production and a 25-fold reduction in the proportion of toxin mRNA to total RNA, was estimated when either 1 mg ml(-1) sorbic acid or 100 microg ml(-1) sodium nitrite were added to the medium at pH 7.0.
竞争性逆转录聚合酶链反应（cRT-PCR）用于定量在含有山梨酸或亚硝酸钠的培养基中肉毒杆菌E型菌株的毒素编码mRNA产量。当在pH 7.0的培养基中添加1 mg/ml山梨酸或100 μg/ml亚硝酸钠时，估计毒素mRNA产量降低了10倍，毒素mRNA占总RNA的比例降低了25倍。
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