Vanbleu Els, Marchal Kathleen, Lambrecht Mark, Mathys Janick, Vanderleyden Jos
Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium.
FEMS Microbiol Lett. 2004 Mar 19;232(2):165-72. doi: 10.1016/S0378-1097(04)00046-1.
The plant growth-promoting soil bacterium Azospirillum brasilense enhances growth of economically important crops, such as wheat, corn and rice. In order to improve plant growth, a close bacterial association with the plant roots is needed. Genes encoded on a 90-MDa plasmid, denoted pRhico plasmid, present in A. brasilense Sp7, play an important role in plant root interaction. Sequencing, annotation and in silico analysis of this 90-MDa plasmid revealed the presence of a large collection of genes encoding enzymes involved in surface polysaccharide biosynthesis. Analysis of the 90-MDa plasmid genome provided evidence for its essential role in the viability of the bacterial cell.
促进植物生长的土壤细菌巴西固氮螺菌可促进小麦、玉米和水稻等重要经济作物的生长。为了促进植物生长，需要细菌与植物根系紧密结合。巴西固氮螺菌Sp7中存在的一个90兆达尔质粒（称为pRhico质粒）上编码的基因在植物根系相互作用中起重要作用。对这个90兆达尔质粒进行测序、注释和计算机分析后发现，有大量编码参与表面多糖生物合成的酶的基因。对90兆达尔质粒基因组的分析证明了它在细菌细胞生存能力方面的重要作用。
