Malbrouck Christelle, Trausch Gérard, Devos Pierre, Kestemont Patrick
Unité de Recherche en Biologie des Organismes, Facultés Universitaires Notre-Dame de la Paix, rue de Bruxelles, 61, B-5000 Namur, Belgium.
Toxicon. 2004 Mar 1;43(3):295-301. doi: 10.1016/j.toxicon.2003.12.007.
Fed and fasted juvenile goldfish Carassius auratus (30 g body weight) were injected intraperitoneally (i.p.) with microcystin-LR (MC-LR) (125 microg/kg body weight) to determine the effect of alimentary status on the hepatic toxicity of MC-LR. The toxin accumulation pattern was similar in both fed and fasted treatments. MC-LR accumulated during the first 48 h post-injection and decreased significantly between 48 and 96 h. MC-LR accumulation induced a decrease in hepatic protein phosphatase activity and glycogen content. Fasted individuals were more severely and more rapidly affected than fed ones. Both indicators were significantly altered after 6 h of fasted treatment. In particular, protein phosphatase activity was totally inhibited after 6 h in the fasted treatment but only lowered and not totally suppressed in the fed one. In both treatments, the recovery of enzyme activity was complete after 96 h. On the other hand, hepatic glutathione concentration and glutathione S-transferase activity were not significantly affected.
给喂食和未喂食的幼年金鱼（体重30克）腹腔注射微囊藻毒素-LR（MC-LR）（125微克/千克体重），以确定营养状态对MC-LR肝毒性的影响。喂食和未喂食处理中，毒素积累模式相似。MC-LR在注射后的前48小时内积累，并在48至96小时之间显著下降。MC-LR积累导致肝蛋白磷酸酶活性和糖原含量降低。未喂食的个体比喂食的个体受到的影响更严重、更迅速。未喂食处理6小时后，两个指标均发生显著变化。特别是，未喂食处理6小时后蛋白磷酸酶活性完全被抑制，但喂食处理中仅降低而未完全抑制。在两种处理中，96小时后酶活性完全恢复。另一方面，肝谷胱甘肽浓度和谷胱甘肽S-转移酶活性未受到显著影响。
