Vengadesan K, Anbupalam T, Gautham N
Department of Crystallography and Biophysics, University of Madras, Chennai 600 025, India.
Biochem Biophys Res Commun. 2004 Apr 9;316(3):731-7. doi: 10.1016/j.bbrc.2004.02.114.
We address the question-can we use experimental design methods to investigate peptide conformation and identify conformational parameters that may contribute more significantly to the potential energy than others? We used mutually orthogonal Latin square design to sample the conformational space of peptides and analysed the samples using analysis of variance. We examined the equality of the effect of the torsion angles on the conformational potential energy. The results showed that different torsion angles contributed differently to the conformational energy. We are able to identify those parameters that may have to be more carefully considered in conformational studies of peptides.
能否使用实验设计方法来研究肽的构象，并识别那些可能比其他参数对势能贡献更为显著的构象参数？我们使用相互正交的拉丁方设计对肽的构象空间进行采样，并使用方差分析对样本进行分析。我们研究了扭转角对构象势能影响的等同性。结果表明，不同的扭转角对构象能量的贡献不同。我们能够识别出在肽的构象研究中可能需要更仔细考虑的那些参数。
