Gorgoulis V, Aninos D, Mikou P, Kanavaros P, Karameris A, Joardanoglou J, Rasidakis A, Veslemes M, Ozanne B, Spandidos D A
Red Cross Hospital, Athens, Greece.
Anticancer Res. 1992 Jul-Aug;12(4):1183-7.
Immunohistochemical study of epidermal growth factor (EGF), epidermal growth factor receptor (EGFR) and transforming growth factor-alpha (TGF-alpha) expression was performed on paraffin-embedded tissue specimens of 70 squamous cell lung carcinomas. The carcinomas were placed to one of the following eight groups, according to the results of EGF, TGF-alpha and EGFR expression: group 1: none, group 2: only EGFR, group 3: EGFR and TGF-alpha, group 4: EGFR and EGF, group 5: TGF-alpha and EGF, group 6: all three, group 7: only TGF-alpha and finally group 8: only EGF. Statistical analysis of the results revealed that the ratio of squamous cell lung carcinomas with lymph node metastasis was significantly higher in groups 4, 5 and 6 (P less than 0.01). We also examined whether EGF receptors were truncated with the use of two monoclonal antibodies directed against different portions of the receptor (EGFR1 and F4). No truncated EGF receptors were detected. These results suggest that lung carcinomas expressing the molecules EGF/EGFR, TFG-alpha/EGFR or TGF/alpha/EGF/EGFR display pathologic features of more aggressive disease.
对70例肺鳞状细胞癌石蜡包埋组织标本进行了表皮生长因子（EGF）、表皮生长因子受体（EGFR）和转化生长因子-α（TGF-α）表达的免疫组织化学研究。根据EGF、TGF-α和EGFR表达结果，将这些癌组织分为以下八组之一：第1组：无表达，第2组：仅EGFR表达，第3组：EGFR和TGF-α表达，第4组：EGFR和EGF表达，第5组：TGF-α和EGF表达，第6组：三者均表达，第7组：仅TGF-α表达，最后第8组：仅EGF表达。结果的统计学分析显示，第4、5和6组中发生淋巴结转移的肺鳞状细胞癌比例显著更高（P<0.01）。我们还使用针对受体不同部分的两种单克隆抗体（EGFR1和F4）检测了EGF受体是否被截断。未检测到截断的EGF受体。这些结果表明，表达EGF/EGFR、TFG-α/EGFR或TGF/α/EGF/EGFR分子的肺癌表现出更具侵袭性疾病的病理特征。
