Miyazaki M, Namba M
Department of Cell Biology, Okayama University Medical School, Japan.
Gan To Kagaku Ryoho. 1992 Aug;19(9):1411-9.
Difficulty in obtaining human liver specimens has made a delay in the development of methodology for isolation and primary culture of human hepatocytes compared to animal hepatocyte cultures. Recently the collagenase-liver-perfusion technique, which was developed for rat hepatocyte isolation, has been applied to prepare isolated human hepatocytes. This method greatly improved the yield and viability of hepatocytes of human liver tissues. Since then, the isolated human hepatocytes and their primary culture have been widely used for studies of hepatic metabolism, regeneration, transplantation, hepatitis B virus infection, hepatotoxins, detection of human carcinogens, hepatocarcinogenesis and so forth. These current studies were reviewed.
与动物肝细胞培养相比，获取人类肝脏标本的困难导致人类肝细胞分离和原代培养方法的开发延迟。最近，为大鼠肝细胞分离而开发的胶原酶肝脏灌注技术已被应用于制备分离的人类肝细胞。该方法极大地提高了人类肝脏组织中肝细胞的产量和活力。从那时起，分离的人类肝细胞及其原代培养已广泛用于肝脏代谢、再生、移植、乙型肝炎病毒感染、肝毒素、人类致癌物检测、肝癌发生等研究。本文对这些当前的研究进行了综述。
