Turesky Robert J, Vouros Paul
Division of Chemistry, National Center for Toxicological Research, 3900 NCTR Road, Jefferson, AR 72079, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Mar 25;802(1):155-66. doi: 10.1016/j.jchromb.2003.10.053.
The detection and quantification of heterocyclic aromatic amine (HAA)-DNA adducts, critical biomarkers in interspecies extrapolation of toxicity data for human risk assessment, remains a challenging analytical problem. The two main analytical methods currently in use to screen for HAA-DNA adducts are the 32P-postlabeling assay and mass spectrometry, using either accelerated mass spectrometry (AMS) or liquid chromatography and electrospray ionization mass spectrometry (LC-ESI-MS). In this review, the principal methods to synthesize and characterize DNA adducts, and the methods applied to measure HAA-DNA adduct in vitro and vivo are discussed.
杂环芳香胺（HAA）-DNA加合物的检测和定量是人类风险评估中毒性数据种间外推的关键生物标志物，仍然是一个具有挑战性的分析问题。目前用于筛查HAA-DNA加合物的两种主要分析方法是32P后标记分析法和质谱分析法，后者使用加速质谱法（AMS）或液相色谱和电喷雾电离质谱法（LC-ESI-MS）。在本综述中，讨论了合成和表征DNA加合物的主要方法，以及用于在体外和体内测量HAA-DNA加合物的方法。
