Li Mengling, Wang Hong, Zhang Xian, Zhang Hua-Shan
Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2004 Mar;60(4):987-93. doi: 10.1016/S1386-1425(03)00329-9.
A new fluorescent probe, 1,3,5,7-tetramethyl-8-(4'-aminophenyl)-4,4-difluoro-4-bora-3a,4a-diaza-s-indacence (TMABODIPY) has been developed for the determination of trace nitrite in terms of the reaction of nitrite with TMABODIPY first in acidic solution and then in alkaline solution to form diazotate, a stable and highly fluorescent reagent. The method offered the advantage of specificity, sensitivity and simplicity. The linear calibration range for nitrite was 8-300 nmol l-1s with a 3 sigma detection limit of 0.65 nmol l-1. The proposed method has been applied to monitor the trace nitrite in drinking water and vegetable without extraction.
一种新型荧光探针，1,3,5,7-四甲基-8-(4'-氨基苯基)-4,4-二氟-4-硼-3a,4a-二氮杂-s-茚（TMABODIPY）已被开发用于测定痕量亚硝酸盐。该方法基于亚硝酸盐先在酸性溶液中与TMABODIPY反应，然后在碱性溶液中反应形成重氮酸盐，重氮酸盐是一种稳定且具有高荧光性的试剂。该方法具有特异性、灵敏度高和操作简单的优点。亚硝酸盐的线性校准范围为8 - 300 nmol l-1，3σ检测限为0.65 nmol l-1。所提出的方法已应用于直接监测饮用水和蔬菜中的痕量亚硝酸盐，无需萃取。
