Kortemme Tanja, Baker David
Howard Hughes Medical Institute and Department of Biochemistry, University of Washington, Box 357350, Seattle, WA 98195, USA.
Curr Opin Chem Biol. 2004 Feb;8(1):91-7. doi: 10.1016/j.cbpa.2003.12.008.
Computational protein design strategies have been developed to reengineer protein-protein interfaces in an automated, generalizable fashion. In the past two years, these methods have been successfully applied to generate chimeric proteins and protein pairs with specificities different from naturally occurring protein-protein interactions. Although there are shortcomings in current approaches, both in the way conformational space is sampled and in the energy functions used to evaluate designed conformations, the successes suggest we are now entering an era in which computational methods can be used to modulate, reengineer and design protein-protein interaction networks in living cells.
计算蛋白质设计策略已被开发出来，用于以自动化、通用的方式重新设计蛋白质-蛋白质界面。在过去两年中，这些方法已成功应用于生成嵌合蛋白和蛋白对，其特异性不同于天然存在的蛋白质-蛋白质相互作用。尽管当前方法存在缺点，无论是在构象空间的采样方式上，还是在用于评估设计构象的能量函数方面，但这些成功表明我们现在正进入一个可以使用计算方法来调节、重新设计和设计活细胞中蛋白质-蛋白质相互作用网络的时代。
