Tassorelli C, Blandini F, Greco R, Nappi G
Laboratory of Pathophysiology of Integrative Autonomic Systems, University Centre for the Study of Adaptive Disorders and Headache (UCADH), IRCCS C. Mondino Institute of Neurology, Pavia, Italy.
J Chem Neuroanat. 2004 Mar;27(1):23-32. doi: 10.1016/j.jchemneu.2003.08.006.
Although the involvement of nitric oxide (NO) in mediating pain and neurovascular coupling is well established, the precise mechanisms sustaining these effects are still unclear. Cyclic GMP (cGMP) probably represents the main effector of the biological effects of NO at the vascular and neuronal levels. Nitroglycerin is a NO donor, which easily crosses the blood brain barrier. Several reports have suggested that the study of nitroglycerin effects upon neuronal and cerebrovascular elements is a useful animal model for investigating the pathophysiological mechanisms underlying migraine. In this study, the anatomic distribution of cGMP in the rat brain was evaluated at serial time-points after systemic administration of nitroglycerin or vehicle. The results show an increase in cGMP immunoreactivity in the nucleus trigeminalis caudalis and in the superficial cortical arterioles 2, 3 and 4h after the drug administration. The data obtained sustains the idea that cGMP is an important mediator of nitroglycerin effect in vascular and neuronal structures that are critical elements for the transmission of cephalic pain.
尽管一氧化氮（NO）参与介导疼痛和神经血管耦合已得到充分证实，但其维持这些效应的精确机制仍不清楚。环磷酸鸟苷（cGMP）可能是NO在血管和神经元水平上产生生物学效应的主要效应分子。硝酸甘油是一种NO供体，它很容易穿过血脑屏障。一些报告表明，研究硝酸甘油对神经元和脑血管成分的影响是一种用于研究偏头痛潜在病理生理机制的有用动物模型。在本研究中，在全身给予硝酸甘油或赋形剂后的连续时间点评估大鼠脑中cGMP的解剖分布。结果显示，给药后2、3和4小时，三叉神经尾核和皮质浅表小动脉中的cGMP免疫反应性增加。获得的数据支持这样一种观点，即cGMP是硝酸甘油在血管和神经元结构中发挥作用的重要介质，而这些结构是头部疼痛传递的关键要素。
