Talieri Maroulio, Papadopoulou Sofia, Scorilas Andreas, Xynopoulos Dimitrios, Arnogianaki Niki, Plataniotis George, Yotis Julia, Agnanti Niki
G. Papanicolau Research Center of Oncology, Saint Savas Hospital, Athens 11522, Greece.
Cancer Lett. 2004 Mar 8;205(1):97-106. doi: 10.1016/j.canlet.2003.09.033.
Lysosomal proteinases, cathepsin B (CB) and cathepsin D (CD) have been implicated in the progression of several human tumors. In the present study, the antigen levels of CB and CD, and their immunohistochemical staining were compared in paired colorectal tumors (n =64) and background colon tissue of the same patients with clinicopathological staging. The antigen levels, were found to be significantly higher in cancer tissue (mean 35.79 ng/mg protein for CB and 3.97 ng/mg protein for CD) than in corresponding normal mucosa (24.62 ng/mg protein for CB and 2.69 ng/mg protein for CD). CB antigen levels were positively correlated with differentiation grade and Duke's stage (P < 0.001 and P = 0.041, respectively), but not correlated with nodal status. CD antigen levels were not correlated with the previous parameters. Staining intensity for both antigens increased from adenoma to adenocarcinoma. The degree of staining for CB and CD was associated with differentiation grade (P = 0.004 and 0.001, respectively), Dukes' stage (P = 0.002 and 0.001, respectively) and lymph node involvement (P = 0.002 and P < 0.001, respectively).
溶酶体蛋白酶组织蛋白酶B（CB）和组织蛋白酶D（CD）与几种人类肿瘤的进展有关。在本研究中，对64例配对的结直肠肿瘤及其同一患者的背景结肠组织进行临床病理分期，比较了CB和CD的抗原水平及其免疫组织化学染色情况。结果发现，癌组织中的抗原水平（CB平均为35.79 ng/mg蛋白质，CD平均为3.97 ng/mg蛋白质）显著高于相应的正常黏膜（CB为24.62 ng/mg蛋白质，CD为2.69 ng/mg蛋白质）。CB抗原水平与分化程度和杜克分期呈正相关（分别为P < 0.001和P = 0.041），但与淋巴结状态无关。CD抗原水平与上述参数无关。两种抗原的染色强度从腺瘤到腺癌均增加。CB和CD的染色程度与分化程度（分别为P = 0.004和0.001）、杜克分期（分别为P = 0.002和0.001）及淋巴结受累情况（分别为P = 0.002和P < 0.001）相关。
