Yu Bo, Li Shi-yong, An Ping, Zuo Fu-yi, Cai Hui-yun
Department of General Surgery, General Hospital of Beijing Military Command, Beijing 100700, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2005 Nov;8(6):507-9.
To investigate the expression of cathepsin B (CatB) in colorectal cancer tissues and serum levels of CatB in patients with colorectal carcinoma and to study the association of CatB expression with lymph node and li ver metastasis.
Immunohistochemistry was used to detect CatB expression in tissues, and enzyme linked immunosorbent assay was applied to test CatB levels in peripheral vein blood in 83 patients with colorectal cancer.
The expression rates of CatB in primary lesions, normal colon mucosa, lymph node metastases and hepatic metastases were 56.6%, 31.3%, 88.4%, 85.0% respectively. The positive rates of CatB in primary lesions, hepatic and lymph node metastases were higher than that in normal mucosa (chi (2)=45.6124, P< 0.01). The CatB expression rates in lymph node and hepatic metastases were higher than that in primary lesions chi (2)=11.5982, 4.3747, P< 0.05). The positive rate of CatB was higher in Dukes C and D tumors than that in Dukes A and B tumors (chi (2)=18.8871, 25.1650, P< 0.01), higher in poorly differentiated and mucous adenocarcinomas than that in well-moderately differentiated adenocarcinomas chi (2)=14.2338, P< 0.05). The mean serum level of CatB in 83 patients with colorectal cancer was (5.9+/- 2.9) ng/ml, higher than (2.3+/- 1.1) ng/ml in the controls of 30 healthy volunteers (t=6.6975, P< 0.01). The serum level of CatB in the patients with Dukes C, D stages were higher than that with Dukes A, B stages.
Enhanced expression of CatB in colorectal cancer tissues is associated with tumor infiltration and metastasis. Monitoring serum CatB level in patients with colorectal cancer is important in the prediction and diagnosis of lymph node and hepatic metastasis,and valuable for evaluation of the therapeutic effect.
探讨组织蛋白酶B（CatB）在结直肠癌组织中的表达及结直肠癌患者血清中CatB水平，研究CatB表达与淋巴结及肝转移的关系。
采用免疫组织化学法检测83例结直肠癌患者组织中CatB的表达，应用酶联免疫吸附测定法检测外周静脉血中CatB水平。
CatB在原发灶、正常结肠黏膜、淋巴结转移灶及肝转移灶中的表达率分别为５６.６％、３１.３％、８８.４％、８５.０％。CatB在原发灶、肝转移灶及淋巴结转移灶中的阳性率高于正常黏膜（χ² ＝ 45.6124，P＜０.０１）。CatB在淋巴结转移灶及肝转移灶中的表达率高于原发灶（χ² ＝ 11.5９８２、4.3747，P＜０.０５）。CatB在Dukes C期和D期肿瘤中的阳性率高于Dukes A期和B期肿瘤（χ² ＝ 18.8871、２5.1650，P＜０.０１），在低分化和黏液腺癌中的阳性率高于高-中分化腺癌（χ² ＝ 14.2338，P＜０.０５）。83例结直肠癌患者血清CatB平均水平为（５.９±２.９）ｎｇ/ml，高于30例健康志愿者对照组的（２.３±１.１）ｎｇ/ml（t ＝ 6.6975，P＜０.０１）。Dukes C期、D期患者血清CatB水平高于Dukes A期、B期患者。
结直肠癌组织中CatB表达增强与肿瘤浸润和转移有关。监测结直肠癌患者血清CatB水平对预测和诊断淋巴结及肝转移具有重要意义，对评估治疗效果也有价值。
