Koike Eiko, Kobayashi Takahiro
Particulate Matter and Diesel Exhaust Project, National Institute for Environmental Studies, Onogawa 16-2, Tsukuba, Ibaraki 305-8506, Japan.
Toxicology. 2004 Mar 15;196(3):217-27. doi: 10.1016/j.tox.2003.10.007.
Ozone (O(3)) as a major component of photochemical air pollutants can increase the levels of allergen-specific antibody and may aggravate allergic symptoms. Antigen presentation is one of the factors contributing to allergic symptoms. Our present study is designed to clarify whether O(3) may increase the antigen-presenting (AP) activity of whole lung cells and its mechanisms. Male Wistar rats were exposed to 1 ppm O(3) for 3 days. The AP activity of whole lung cells and dendritic cells (DC) was measured by proliferation of T-cells. The expression of Ia and costimulatory molecules (B7.1, B7.2, CD11b/c) in lung cells was measured by flow cytometry, and the number of Ia-bearing cells, DC, macrophages, and B-cells in lung interstitum was examined immunohistochemically. The results show that O(3) increases AP activity of whole lung cells and DC, the expression of molecules associated with antigen presentation, and the number of AP cells (APC) in lung. Our results suggest that O(3) may enhance AP activity of lung cells caused by increases in the expression of cell-surface molecules and the number of APC in lung. The increase in the AP activity might contribute to subsequent antibody production, airway hyperresponsiveness and aggravation of allergic responses.
臭氧（O(3)）作为光化学空气污染物的主要成分，可增加变应原特异性抗体水平，并可能加重过敏症状。抗原呈递是导致过敏症状的因素之一。我们目前的研究旨在阐明O(3)是否会增加全肺细胞的抗原呈递（AP）活性及其机制。将雄性Wistar大鼠暴露于1 ppm的O(3)中3天。通过T细胞增殖来测量全肺细胞和树突状细胞（DC）的AP活性。通过流式细胞术测量肺细胞中Ia和共刺激分子（B7.1、B7.2、CD11b/c）的表达，并通过免疫组织化学检查肺间质中Ia阳性细胞、DC、巨噬细胞和B细胞的数量。结果表明，O(3)可增加全肺细胞和DC的AP活性、与抗原呈递相关分子的表达以及肺中AP细胞（APC）的数量。我们的结果表明，O(3)可能通过增加肺细胞表面分子的表达和APC的数量来增强肺细胞的AP活性。AP活性的增加可能有助于随后的抗体产生、气道高反应性和过敏反应的加重。
