Frasca Daniela, Nguyen Diep, Riley Richard L, Blomberg Bonnie B
Department of Microbiology and Immunology, University of Miami School of Medicine, 1600 N.W. 10th Ave, Miami, FL 33136, USA.
Mech Ageing Dev. 2004 Feb;125(2):111-2. doi: 10.1016/j.mad.2003.11.004.
In the present paper, we have investigated the DNA-binding activity of the E2A-encoded transcription factor E47 in LPS-activated splenic B cells from young and old BALB/c mice. E47 is a key regulator of B cell differentiation and function: it binds to the E-box site, found in the regulatory regions of several B cell-specific genes (immunoglobulin (Ig), mb-1, and lambda5), promotes cell survival of early pre-B cells, helps to initiate Ig rearrangements, promotes class switch and is perhaps involved in somatic mutation in mature splenic and lymph node B cells. Results show that LPS-stimulated splenic B cells from old mice display decreased E47 DNA-binding activity as compared to young mice and that in splenic activated B cells only E47 homodimers bind DNA.
在本论文中，我们研究了E2A编码的转录因子E47在来自年轻和年老BALB/c小鼠的LPS激活的脾B细胞中的DNA结合活性。E47是B细胞分化和功能的关键调节因子：它与在几个B细胞特异性基因（免疫球蛋白（Ig）、mb-1和lambda5）的调控区域中发现的E盒位点结合，促进早期前B细胞的细胞存活，有助于启动Ig重排，促进类别转换，并且可能参与成熟脾和淋巴结B细胞中的体细胞突变。结果表明，与年轻小鼠相比，来自年老小鼠的LPS刺激的脾B细胞显示出降低的E47 DNA结合活性，并且在脾活化B细胞中只有E47同源二聚体结合DNA。
