Clamp Michael F, Ochrietor Judith D, Moroz Tatiana P, Linser Paul J
The Whitney Laboratory, University of Florida, 9505 Ocean Shore Boulevard, St Augustine, FL 32080, USA.
Exp Eye Res. 2004 Apr;78(4):777-89. doi: 10.1016/j.exer.2003.12.004.
Recent reports by this laboratory and others have demonstrated an association between 5A11/Basigin, a member of the immunoglobulin gene superfamily, and monocarboxylate transporter-1 (MCT1), a lactose transporter. Indeed, it was determined in the 5A11/Basigin null mouse retina that MCT1 does not properly integrate into the cell membranes of Müller cells (MCs) or the retinal-pigmented epithelium, where the two are colocalized. The purpose of this study was to elucidate the association of 5A11/Basigin and MCT1 in the developing mouse retina. Immunocytochemical localization and real-time RT-PCR were used to evaluate the expression and localization of 5A11/Basigin and MCT1 at embryonic days 12, 15, and 18, as well as post-natal days 1, 7, 14, and 21. Expression of both proteins progressed from a more generalized distribution throughout the undifferentiated neural retina to specific staining of retina-pigmented epithilia, the MCs, photoreceptor cells and the ciliary apparatus. Although these two membrane glycoproteins were often colocalized, distinct differences in the location and magnitude of their expression over time was observed. These findings suggest that although 5A11/Basigin and MCT1 can associate within the cell membrane, their expression is not always associated and colocalized.
本实验室及其他机构最近的报告表明，免疫球蛋白基因超家族成员5A11/基底膜蛋白（Basigin）与乳糖转运体单羧酸转运蛋白-1（MCT1）之间存在关联。实际上，在5A11/基底膜蛋白基因敲除小鼠的视网膜中已确定，MCT1无法正常整合到米勒细胞（MCs）或视网膜色素上皮细胞的细胞膜中，而这两种细胞中MCT1是共定位的。本研究的目的是阐明发育中小鼠视网膜中5A11/基底膜蛋白与MCT1的关联。采用免疫细胞化学定位和实时逆转录聚合酶链反应（RT-PCR）来评估5A11/基底膜蛋白和MCT1在胚胎第12、15和18天以及出生后第1、7、14和21天的表达和定位。两种蛋白的表达从在未分化的神经视网膜中更广泛的分布发展为在视网膜色素上皮细胞、米勒细胞、光感受器细胞和睫状体装置中的特异性染色。尽管这两种膜糖蛋白经常共定位，但随着时间推移，观察到它们在位置和表达量上存在明显差异。这些发现表明，尽管5A11/基底膜蛋白和MCT1可在细胞膜内发生关联，但其表达并不总是相关且共定位的。
