• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

低氧诱导的小胶质细胞中上调的 basigin-2 促进视网膜血管生成。

Up-regulated basigin-2 in microglia induced by hypoxia promotes retinal angiogenesis.

机构信息

Department of Ophthalmology, Eye Institute of China PLA, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

Department of Ophthalmology, Jinling Hospital, Nanjing, China.

出版信息

J Cell Mol Med. 2017 Dec;21(12):3467-3480. doi: 10.1111/jcmm.13256. Epub 2017 Jun 29.

DOI:10.1111/jcmm.13256
PMID:28661035
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5706566/
Abstract

Retinal microglia cells contribute to vascular angiogenesis and vasculopathy induced by relative hypoxia. However, its concrete molecular mechanisms in shaping retinal angiogenesis have not been elucidated. Basigin, being involved in tumour neovasculogenesis, is explored to exert positive effects on retinal angiogenesis induced by microglia. Therefore, we set out to investigate the expression of basigin using a well-characterized mouse model of oxygen-induced retinopathy, which recapitulated hypoxia-induced aberrant neovessel growth. Our results elucidate that basigin is overexpressed in microglia, which accumulating in retinal angiogenic sprouts. In vitro, conditioned media from microglia BV2 under hypoxia treatment increase migration and tube formation of retinal capillary endothelia cells, compared with media from normoxic condition. The angiogenic capacity of BV2 is inhibited after basigin knockdown by small interfering RNAs. A new molecular mechanism for high angiogenic capacity, whereby microglia cells release basigin via up-regulation of PI3K-AKT and IGF-1 pathway to induce angiogenesis is unveiled. Collectively, our results demonstrate that basigin from hypoxic microglia plays a pivotal pro-angiogenic role, providing new insights into microglia-promoting retinal angiogenesis.

摘要

视网膜小胶质细胞有助于相对缺氧引起的血管生成和血管病变。然而，其在塑造视网膜血管生成方面的具体分子机制尚未阐明。Basigin 参与肿瘤新生血管生成，被探索对小胶质细胞诱导的视网膜血管生成发挥积极作用。因此，我们使用一种经过充分表征的氧诱导视网膜病变小鼠模型来研究 basigin 的表达，该模型重现了缺氧诱导的异常新生血管生长。我们的结果阐明了 basigin 在小胶质细胞中过度表达，这些小胶质细胞聚集在视网膜血管生成芽中。在体外，与正常氧条件下的培养基相比，缺氧处理的小胶质细胞 BV2 的条件培养基增加了视网膜毛细血管内皮细胞的迁移和管形成。用小干扰 RNA 敲低 basigin 后，BV2 的血管生成能力受到抑制。揭示了一种新的高血管生成能力的分子机制，即小胶质细胞通过上调 PI3K-AKT 和 IGF-1 通路释放 basigin 来诱导血管生成。总之，我们的结果表明，缺氧小胶质细胞中的 basigin 发挥着关键的促血管生成作用，为小胶质细胞促进视网膜血管生成提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a454/5706566/6a0488ab07ea/JCMM-21-3467-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a454/5706566/2fdd40e2afdf/JCMM-21-3467-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a454/5706566/844df2a2377e/JCMM-21-3467-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a454/5706566/c258d4715075/JCMM-21-3467-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a454/5706566/b6ed99c1dd4a/JCMM-21-3467-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a454/5706566/26eb328a9d13/JCMM-21-3467-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a454/5706566/ea0cdcc3bfcb/JCMM-21-3467-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a454/5706566/6a0488ab07ea/JCMM-21-3467-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a454/5706566/2fdd40e2afdf/JCMM-21-3467-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a454/5706566/844df2a2377e/JCMM-21-3467-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a454/5706566/c258d4715075/JCMM-21-3467-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a454/5706566/b6ed99c1dd4a/JCMM-21-3467-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a454/5706566/26eb328a9d13/JCMM-21-3467-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a454/5706566/ea0cdcc3bfcb/JCMM-21-3467-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a454/5706566/6a0488ab07ea/JCMM-21-3467-g007.jpg

相似文献

1
Up-regulated basigin-2 in microglia induced by hypoxia promotes retinal angiogenesis.低氧诱导的小胶质细胞中上调的 basigin-2 促进视网膜血管生成。
J Cell Mol Med. 2017 Dec;21(12):3467-3480. doi: 10.1111/jcmm.13256. Epub 2017 Jun 29.
2
Basic fibroblast growth factor contributes to a shift in the angioregulatory activity of retinal glial (Müller) cells.碱性成纤维细胞生长因子有助于改变视网膜神经胶质（Müller）细胞的血管生成调节活性。
PLoS One. 2013 Jul 4;8(7):e68773. doi: 10.1371/journal.pone.0068773. Print 2013.
3
Down-regulation of microRNA-155 attenuates retinal neovascularization via the PI3K/Akt pathway.微小RNA-155的下调通过PI3K/Akt信号通路减轻视网膜新生血管形成。
Mol Vis. 2015 Oct 13;21:1173-84. eCollection 2015.
4
Deletion of Tgfβ signal in activated microglia prolongs hypoxia-induced retinal neovascularization enhancing Igf1 expression and retinal leukostasis.激活的小胶质细胞中 TGFβ 信号的缺失延长了低氧诱导的视网膜新生血管形成，增强了 Igf1 的表达和视网膜白细胞淤滞。
Glia. 2022 Sep;70(9):1762-1776. doi: 10.1002/glia.24218. Epub 2022 May 25.
5
LGALS3BP in Microglia Promotes Retinal Angiogenesis Through PI3K/AKT Pathway During Hypoxia.LGALS3BP 在低氧条件下通过 PI3K/AKT 通路促进小胶质细胞中的视网膜血管生成。
Invest Ophthalmol Vis Sci. 2022 Jul 8;63(8):25. doi: 10.1167/iovs.63.8.25.
6
Autophagy is required for the promoting effect of angiogenic factor with G patch domain and forkhead-associated domain 1 (AGGF1) in retinal angiogenesis.自噬对于血管生成因子 G 补丁结构域和叉头相关结构域 1（AGGF1）在视网膜血管生成中的促进作用是必需的。
Microvasc Res. 2021 Nov;138:104230. doi: 10.1016/j.mvr.2021.104230. Epub 2021 Jul 31.
7
Single and Compound Knock-outs of MicroRNA (miRNA)-155 and Its Angiogenic Gene Target CCN1 in Mice Alter Vascular and Neovascular Growth in the Retina via Resident Microglia.小鼠中MicroRNA（miRNA）-155及其血管生成基因靶点CCN1的单基因和复合基因敲除通过视网膜驻留小胶质细胞改变视网膜血管和新生血管的生长。
J Biol Chem. 2015 Sep 18;290(38):23264-81. doi: 10.1074/jbc.M115.646950. Epub 2015 Aug 4.
8
GBP2 inhibits pathological angiogenesis in the retina via the AKT/mTOR/VEGFA axis.GBP2 通过 AKT/mTOR/VEGFA 轴抑制视网膜病理性血管生成。
Microvasc Res. 2024 Jul;154:104689. doi: 10.1016/j.mvr.2024.104689. Epub 2024 Apr 16.
9
Antioxidative effects of polypyrimidine tract-binding protein-associated splicing factor against pathological retinal angiogenesis through promotion of mitochondrial function.嘧啶重复序列结合蛋白相关剪接因子通过促进线粒体功能对病理性视网膜血管生成的抗氧化作用。
J Mol Med (Berl). 2021 Jul;99(7):967-980. doi: 10.1007/s00109-021-02069-z. Epub 2021 Mar 26.
10
CCN1/Cyr61-PI3K/AKT signaling promotes retinal neovascularization in oxygen-induced retinopathy.CCN1/ Cyr61 - PI3K/AKT信号通路促进氧诱导性视网膜病变中的视网膜新生血管形成。
Int J Mol Med. 2015 Dec;36(6):1507-18. doi: 10.3892/ijmm.2015.2371. Epub 2015 Oct 12.

引用本文的文献

1
Understanding amblyopia from the perspective of neurovascular units: changes in the retina and brain.从神经血管单元的角度理解弱视：视网膜和大脑的变化。
Front Cell Dev Biol. 2025 Jun 27;13:1590009. doi: 10.3389/fcell.2025.1590009. eCollection 2025.
2
The role of microglia in the development of diabetic retinopathy and its potential clinical application.小胶质细胞在糖尿病视网膜病变发展中的作用及其潜在的临床应用。
Hum Cell. 2025 May 20;38(4):101. doi: 10.1007/s13577-025-01226-7.
3
Non-histone lactylation: unveiling its functional significance.

本文引用的文献

1
POSTNATAL SERUM INSULIN-LIKE GROWTH FACTOR I AND RETINOPATHY OF PREMATURITY.产后血清胰岛素样生长因子I与早产儿视网膜病变
Retina. 2017 May;37(5):867-872. doi: 10.1097/IAE.0000000000001247.
2
Time lapse in vivo microscopy reveals distinct dynamics of microglia-tumor environment interactions-a new role for the tumor perivascular space as highway for trafficking microglia.实时体内显微镜检查揭示了小胶质细胞与肿瘤环境相互作用的独特动态——肿瘤血管周围间隙作为小胶质细胞运输通道的新作用。
Glia. 2016 Jul;64(7):1210-26. doi: 10.1002/glia.22994. Epub 2016 May 3.
3
The altered glucose metabolism in tumor and a tumor acidic microenvironment associated with extracellular matrix metalloproteinase inducer and monocarboxylate transporters.
非组蛋白乳酰化：揭示其功能意义。
Front Cell Dev Biol. 2025 Jan 24;13:1535611. doi: 10.3389/fcell.2025.1535611. eCollection 2025.
4
Spinal Cord Injury Management Based on Microglia-Targeting Therapies.基于小胶质细胞靶向治疗的脊髓损伤管理
J Clin Med. 2024 May 8;13(10):2773. doi: 10.3390/jcm13102773.
5
Glucose Promotes EMMPRIN/CD147 and the Secretion of Pro-Angiogenic Factors in a Co-Culture System of Endothelial Cells and Monocytes.葡萄糖在内皮细胞与单核细胞共培养体系中促进细胞外基质金属蛋白酶诱导因子/CD147及促血管生成因子的分泌。
Biomedicines. 2024 Mar 22;12(4):706. doi: 10.3390/biomedicines12040706.
6
Microglia in retinal angiogenesis and diabetic retinopathy.视网膜血管生成和糖尿病视网膜病变中的小胶质细胞。
Angiogenesis. 2024 Aug;27(3):311-331. doi: 10.1007/s10456-024-09911-1. Epub 2024 Apr 2.
7
ω-3PUFAs Inhibit Hypoxia-Induced Retinal Neovascularization via Regulating Microglial Pyroptosis through METTL14-Mediated m6A Modification of IFNB1 mRNA.ω-3PUFAs 通过调节 METTL14 介导的 IFNB1 mRNA m6A 修饰抑制缺氧诱导的视网膜新生血管形成通过小胶质细胞焦亡。
Appl Biochem Biotechnol. 2024 Sep;196(9):5936-5952. doi: 10.1007/s12010-023-04795-1. Epub 2024 Jan 4.
8
Microglial Galectin3 enhances endothelial metabolism and promotes pathological angiogenesis via Notch inhibition by competitively binding to Jag1.小胶质细胞半乳糖凝集素 3 通过与 Jag1 竞争结合来抑制 Notch，从而增强内皮细胞代谢并促进病理性血管生成。
Cell Death Dis. 2023 Jun 28;14(6):380. doi: 10.1038/s41419-023-05897-8.
9
Microglia-Derived Spp1 Promotes Pathological Retinal Neovascularization via Activating Endothelial Kit/Akt/mTOR Signaling.小胶质细胞衍生的Spp1通过激活内皮细胞Kit/Akt/mTOR信号通路促进病理性视网膜新生血管形成。
J Pers Med. 2023 Jan 11;13(1):146. doi: 10.3390/jpm13010146.
10
Can CD147 work as a therapeutic target for tumors through COVID-19 infection?CD147 能否通过 COVID-19 感染成为肿瘤的治疗靶点？
Int J Med Sci. 2022 Nov 21;19(14):2087-2092. doi: 10.7150/ijms.79162. eCollection 2022.
肿瘤中改变的葡萄糖代谢以及与细胞外基质金属蛋白酶诱导剂和单羧酸转运蛋白相关的肿瘤酸性微环境。
Oncotarget. 2016 Apr 26;7(17):23141-55. doi: 10.18632/oncotarget.8153.
4
Myeloid-Derived Vascular Endothelial Growth Factor and Hypoxia-Inducible Factor Are Dispensable for Ocular Neovascularization--Brief Report.髓源性血管内皮生长因子和缺氧诱导因子对眼新生血管形成并非必需——简要报告
Arterioscler Thromb Vasc Biol. 2016 Jan;36(1):19-24. doi: 10.1161/ATVBAHA.115.306681. Epub 2015 Nov 24.
5
Cell metabolism: Sugar for sight.细胞代谢：视觉所需的糖分。
Nature. 2015 Jun 25;522(7557):428-9. doi: 10.1038/522428a.
6
Hypoxia, cancer metabolism and the therapeutic benefit of targeting lactate/H(+) symporters.缺氧、癌症代谢与靶向乳酸/H(+) 同向转运体的治疗益处
J Mol Med (Berl). 2016 Feb;94(2):155-71. doi: 10.1007/s00109-015-1307-x. Epub 2015 Jun 24.
7
Rod-derived cone viability factor promotes cone survival by stimulating aerobic glycolysis.杆状细胞衍生视锥细胞生存因子通过刺激有氧糖酵解促进视锥细胞存活。
Cell. 2015 May 7;161(4):817-32. doi: 10.1016/j.cell.2015.03.023.
8
EMMPRIN/CD147 is a novel coreceptor of VEGFR-2 mediating its activation by VEGF.细胞外基质金属蛋白酶诱导因子/CD147是血管内皮生长因子受体-2（VEGFR-2）的一种新型共受体，介导其被血管内皮生长因子（VEGF）激活。
Oncotarget. 2015;6(12):9766-80. doi: 10.18632/oncotarget.2870.
9
Functional relevance of protein glycosylation to the pro-inflammatory effects of extracellular matrix metalloproteinase inducer (EMMPRIN) on monocytes/macrophages.蛋白质糖基化与细胞外基质金属蛋白酶诱导剂（EMMPRIN）对单核细胞/巨噬细胞的促炎作用的功能相关性。
PLoS One. 2015 Feb 6;10(2):e0117463. doi: 10.1371/journal.pone.0117463. eCollection 2015.
10
Pharmacokinetics of bevacizumab and its effects on serum VEGF and IGF-1 in infants with retinopathy of prematurity.早产儿视网膜病变患儿贝伐单抗的药代动力学及其对血清 VEGF 和 IGF-1 的影响。
Invest Ophthalmol Vis Sci. 2015 Jan 22;56(2):956-61. doi: 10.1167/iovs.14-15842.