A general, PCR-based method for single or combinatorial oligonucleotide-directed mutagenesis on pUC/M13 vectors.

Suppr

超能文献

作者信息

Merino E, Osuna J, Bolívar F, Soberón X

机构信息

Instituto de Biotecnología, UNAM, Cuernavaca, Morelos, Mexico.

出版信息

Biotechniques. 1992 Apr;12(4):508-10.

PMID:1503750

Abstract

摘要

相似文献

A general, PCR-based method for single or combinatorial oligonucleotide-directed mutagenesis on pUC/M13 vectors.

Biotechniques. 1992 Apr;12(4):508-10.

In vivo site-directed mutagenesis using oligonucleotides.使用寡核苷酸进行体内定点诱变。

Nat Biotechnol. 2001 Aug;19(8):773-6. doi: 10.1038/90837.

Site-directed mutagenesis using a rapid PCR-based method.使用基于快速聚合酶链式反应的方法进行定点诱变。

Methods Mol Biol. 1996;57:239-48. doi: 10.1385/0-89603-332-5:239.

Using PCR for rapid site-specific mutagenesis in large plasmids.利用聚合酶链反应（PCR）对大型质粒进行快速位点特异性诱变。

Methods Mol Biol. 1996;57:217-27. doi: 10.1385/0-89603-332-5:217.

Recombinant circle PCR and recombination PCR for site-specific mutagenesis without PCR product purification.无需纯化PCR产物的用于位点特异性诱变的重组环PCR和重组PCR。

Biotechniques. 1992 Apr;12(4):528-30, 532, 534-5.

PCR-based site-directed mutagenesis.基于聚合酶链反应的定点诱变

Methods Mol Biol. 1996;57:157-65. doi: 10.1385/0-89603-332-5:157.

An oligonucleotide-directed, in vitro mutagenesis method using ssDNA and preferential DNA amplification of the mutated strand.一种使用单链DNA的寡核苷酸定向体外诱变方法及突变链的优先DNA扩增。

Biotechniques. 1994 Oct;17(4):719-20, 722, 724-5.

Generation of multiple mutations in the same sequence via the polymerase chain reaction using a single selection primer.

Anal Biochem. 1994 Sep;221(2):425-8. doi: 10.1006/abio.1994.1440.

Site-specific mutagenesis by using an accurate recombinant polymerase chain reaction method.使用精确重组聚合酶链反应方法进行位点特异性诱变。

Anal Biochem. 1996 Feb 1;234(1):110-1. doi: 10.1006/abio.1996.0060.

[Oligonucleotide directed in vitro mutagenesis].[寡核苷酸定向体外诱变]

Wei Sheng Wu Xue Bao. 1993 Oct;33(5):331-8.

引用本文的文献

The effect of amino acid deletions and substitutions in the longest loop of GFP.绿色荧光蛋白最长环中氨基酸缺失和替换的影响。

BMC Chem Biol. 2007 Jun 26;7:1. doi: 10.1186/1472-6769-7-1.

DNA repair and transcriptional deficiencies caused by mutations in the Drosophila p52 subunit of TFIIH generate developmental defects and chromosome fragility.果蝇TFIIH的p52亚基发生突变导致的DNA修复和转录缺陷会产生发育缺陷和染色体脆性。

Mol Cell Biol. 2007 May;27(10):3640-50. doi: 10.1128/MCB.00030-07. Epub 2007 Mar 5.

DNA repair and transcriptional effects of mutations in TFIIH in Drosophila development.果蝇发育过程中TFIIH突变的DNA修复和转录效应。

Mol Biol Cell. 2002 Sep;13(9):3246-56. doi: 10.1091/mbc.e02-02-0087.

Novel ceftazidime-resistance beta-lactamases generated by a codon-based mutagenesis method and selection.通过基于密码子的诱变方法和筛选产生的新型耐头孢他啶β-内酰胺酶。

Nucleic Acids Res. 2002 Aug 15;30(16):e84. doi: 10.1093/nar/gnf083.

The hydrogenase cytochrome b heme ligands of Azotobacter vinelandii are required for full H(2) oxidation capability.棕色固氮菌的氢化酶细胞色素b血红素配体是完全具备H₂氧化能力所必需的。

J Bacteriol. 2000 Jun;182(12):3429-36. doi: 10.1128/JB.182.12.3429-3436.2000.

In vivo genomic footprinting analysis reveals that the complex Bradyrhizobium japonicum fixRnifA promoter region is differently occupied by two distinct RNA polymerase holoenzymes.体内基因组足迹分析表明，复杂的日本慢生根瘤菌fixRnifA启动子区域被两种不同的RNA聚合酶全酶以不同方式占据。

Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1014-9. doi: 10.1073/pnas.95.3.1014.

Functional and structural analysis of the sialic acid-binding domain of rotaviruses.轮状病毒唾液酸结合结构域的功能与结构分析

J Virol. 1997 Sep;71(9):6749-56. doi: 10.1128/JVI.71.9.6749-6756.1997.

Nucleotide sequences and regulational analysis of genes involved in conversion of aniline to catechol in Pseudomonas putida UCC22(pTDN1).恶臭假单胞菌UCC22(pTDN1)中参与苯胺转化为儿茶酚的基因的核苷酸序列及调控分析。

J Bacteriol. 1997 Jan;179(2):399-408. doi: 10.1128/jb.179.2.399-408.1997.

Dissection of the transcription machinery for housekeeping genes of Bradyrhizobium japonicum.慢生根瘤菌持家基因转录机制的剖析。

J Bacteriol. 1997 Jan;179(2):364-9. doi: 10.1128/jb.179.2.364-369.1997.

EBF contains a novel zinc coordination motif and multiple dimerization and transcriptional activation domains.EBF含有一个新型锌配位基序以及多个二聚化和转录激活结构域。

EMBO J. 1995 Jun 15;14(12):2907-16. doi: 10.1002/j.1460-2075.1995.tb07290.x.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验