从“不纯”DNA中进行双链双脱氧测序——一天内完成。
Double-stranded dideoxy sequencing from "dirty" DNA--done in a day.
作者信息
Disotell T R, Zehr S M, Ruvolo M
机构信息
Department of Anthropology, Harvard University, Cambridge, MA 02138.
出版信息
Biotechniques. 1992 Jul;13(1):48-50.
PMID:1503774
Abstract
A rapid method for preparing and directly sequencing plasmid and phagemid miniprep DNA is described. This protocol is a novel combination of two fairly standard procedures, resulting in quick and easy generation of sequence data. The lack of extensive manipulations in the purification process allows the production of DNA sequence data in a single day.
摘要
本文描述了一种制备质粒和噬菌粒小量制备DNA并直接进行测序的快速方法。该方案是两种相当标准的程序的新颖组合，能够快速简便地生成序列数据。纯化过程中无需大量操作，可在一天内产生DNA序列数据。
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