• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Mutations in the middle domain of yeast poly(A) polymerase affect interactions with RNA but not ATP.酵母聚腺苷酸聚合酶中间结构域的突变会影响其与RNA的相互作用，但不影响与ATP的相互作用。
RNA. 2004 Apr;10(4):558-64. doi: 10.1261/rna.5238704.
2
Mechanism of poly(A) polymerase: structure of the enzyme-MgATP-RNA ternary complex and kinetic analysis.聚腺苷酸聚合酶的作用机制：酶-MgATP-RNA三元复合物的结构及动力学分析
Structure. 2007 Sep;15(9):1117-31. doi: 10.1016/j.str.2007.07.010.
3
Structure of yeast poly(A) polymerase alone and in complex with 3'-dATP.单独的酵母聚腺苷酸聚合酶及其与3'-dATP复合物的结构。
Science. 2000 Aug 25;289(5483):1346-9. doi: 10.1126/science.289.5483.1346.
4
A sensitive, single-tube assay to measure the enzymatic activities of influenza RNA polymerase and other poly(A) polymerases: application to kinetic and inhibitor analysis.一种用于测量流感病毒RNA聚合酶和其他聚腺苷酸聚合酶酶活性的灵敏单管检测方法：应用于动力学和抑制剂分析。
Nucleic Acids Res. 2001 Jul 1;29(13):2691-8. doi: 10.1093/nar/29.13.2691.
5
Involvement of the carboxyl terminus of vertebrate poly(A) polymerase in U1A autoregulation and in the coupling of splicing and polyadenylation.脊椎动物聚腺苷酸聚合酶的羧基末端在U1A自身调节以及剪接与聚腺苷酸化偶联中的作用。
Genes Dev. 1997 Mar 15;11(6):761-73. doi: 10.1101/gad.11.6.761.
6
Structure-function relationships in the Saccharomyces cerevisiae poly(A) polymerase. Identification of a novel RNA binding site and a domain that interacts with specificity factor(s).酿酒酵母多聚腺苷酸聚合酶中的结构-功能关系。一个新的RNA结合位点和一个与特异性因子相互作用的结构域的鉴定。
J Biol Chem. 1995 Nov 3;270(44):26715-20. doi: 10.1074/jbc.270.44.26715.
7
Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins involved in mRNA degradation.大肠杆菌的聚腺苷酸聚合酶I：催化结构域、RNA结合位点以及与参与mRNA降解的蛋白质相互作用区域的特性
Mol Microbiol. 1999 May;32(4):765-75. doi: 10.1046/j.1365-2958.1999.01394.x.
8
Proton transfer in the mechanism of polyadenylate polymerase.聚腺苷酸聚合酶机制中的质子转移
Biochem J. 2009 May 13;420(2):229-38. doi: 10.1042/BJ20082019.
9
Biochemical and structural insights into substrate binding and catalytic mechanism of mammalian poly(A) polymerase.对哺乳动物多聚腺苷酸聚合酶底物结合和催化机制的生化及结构见解。
J Mol Biol. 2004 Aug 20;341(4):911-25. doi: 10.1016/j.jmb.2004.06.047.
10
X-ray crystallographic and steady state fluorescence characterization of the protein dynamics of yeast polyadenylate polymerase.酵母聚腺苷酸聚合酶蛋白质动力学的X射线晶体学和稳态荧光表征
J Mol Biol. 2007 Mar 9;366(5):1401-15. doi: 10.1016/j.jmb.2006.12.030. Epub 2006 Dec 19.

引用本文的文献

1
The role of sequence context, nucleotide pool balance and stress in 2'-deoxynucleotide misincorporation in viral, bacterial and mammalian RNA.序列上下文、核苷酸库平衡和应激在病毒、细菌及哺乳动物RNA中2'-脱氧核苷酸错掺入中的作用
Nucleic Acids Res. 2016 Oct 14;44(18):8962-8975. doi: 10.1093/nar/gkw572. Epub 2016 Jun 30.
2
Mechanism of poly(A) polymerase: structure of the enzyme-MgATP-RNA ternary complex and kinetic analysis.聚腺苷酸聚合酶的作用机制：酶-MgATP-RNA三元复合物的结构及动力学分析
Structure. 2007 Sep;15(9):1117-31. doi: 10.1016/j.str.2007.07.010.
3
Dual role of the RNA substrate in selectivity and catalysis by terminal uridylyl transferases.RNA底物在末端尿苷酰转移酶的选择性和催化作用中的双重作用。
Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14634-9. doi: 10.1073/pnas.0704259104. Epub 2007 Sep 4.
4
X-ray crystallographic and steady state fluorescence characterization of the protein dynamics of yeast polyadenylate polymerase.酵母聚腺苷酸聚合酶蛋白质动力学的X射线晶体学和稳态荧光表征
J Mol Biol. 2007 Mar 9;366(5):1401-15. doi: 10.1016/j.jmb.2006.12.030. Epub 2006 Dec 19.
5
UTP-bound and Apo structures of a minimal RNA uridylyltransferase.一种最小RNA尿苷酰转移酶的UTP结合结构和脱辅基结构。
J Mol Biol. 2007 Feb 23;366(3):882-99. doi: 10.1016/j.jmb.2006.11.065. Epub 2006 Dec 2.

本文引用的文献

1
A history of poly A sequences: from formation to factors to function.多聚腺苷酸序列的历史：从形成到相关因子再到功能
Prog Nucleic Acid Res Mol Biol. 2002;71:285-389. doi: 10.1016/s0079-6603(02)71046-5.
2
Crystal structures of a template-independent DNA polymerase: murine terminal deoxynucleotidyltransferase.一种不依赖模板的DNA聚合酶——小鼠末端脱氧核苷酸转移酶的晶体结构
EMBO J. 2002 Feb 1;21(3):427-39. doi: 10.1093/emboj/21.3.427.
3
Fip1 regulates the activity of Poly(A) polymerase through multiple interactions.Fip1通过多种相互作用调节聚腺苷酸聚合酶的活性。
Mol Cell Biol. 2001 Mar;21(6):2026-37. doi: 10.1128/MCB.21.6.2026-2037.2001.
4
Structure of yeast poly(A) polymerase alone and in complex with 3'-dATP.单独的酵母聚腺苷酸聚合酶及其与3'-dATP复合物的结构。
Science. 2000 Aug 25;289(5483):1346-9. doi: 10.1126/science.289.5483.1346.
5
Crystal structure of mammalian poly(A) polymerase in complex with an analog of ATP.与ATP类似物结合的哺乳动物聚腺苷酸聚合酶的晶体结构。
EMBO J. 2000 Aug 15;19(16):4193-203. doi: 10.1093/emboj/19.16.4193.
6
Mapping of ATP binding regions in poly(A) polymerases by photoaffinity labeling and by mutational analysis identifies a domain conserved in many nucleotidyltransferases.通过光亲和标记和突变分析对聚腺苷酸聚合酶中ATP结合区域进行定位，确定了在许多核苷酸转移酶中保守的一个结构域。
Protein Sci. 1999 Nov;8(11):2380-91. doi: 10.1110/ps.8.11.2380.
7
An open and closed case for all polymerases.关于所有聚合酶的一个公开与已了结的案例。
Structure. 1999 Feb 15;7(2):R31-5. doi: 10.1016/S0969-2126(99)80017-3.
8
Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.真核生物中mRNA 3'末端的形成：机制、调控及其与mRNA合成其他步骤的相互关系
Microbiol Mol Biol Rev. 1999 Jun;63(2):405-45. doi: 10.1128/MMBR.63.2.405-445.1999.
9
Processivity of the Saccharomyces cerevisiae poly(A) polymerase requires interactions at the carboxyl-terminal RNA binding domain.酿酒酵母多聚腺苷酸聚合酶的持续合成能力需要在羧基末端RNA结合结构域进行相互作用。
Mol Cell Biol. 1998 Oct;18(10):5942-51. doi: 10.1128/MCB.18.10.5942.
10
CCA-adding enzymes and poly(A) polymerases are all members of the same nucleotidyltransferase superfamily: characterization of the CCA-adding enzyme from the archaeal hyperthermophile Sulfolobus shibatae.添加CCA的酶和聚腺苷酸聚合酶都是同一核苷酸转移酶超家族的成员：来自嗜热古菌柴田硫化叶菌的添加CCA的酶的特性
RNA. 1996 Sep;2(9):895-908.

酵母聚腺苷酸聚合酶中间结构域的突变会影响其与RNA的相互作用，但不影响与ATP的相互作用。

Mutations in the middle domain of yeast poly(A) polymerase affect interactions with RNA but not ATP.

作者信息

Zhelkovsky Alexander, Helmling Steffen, Bohm Andrew, Moore Claire

机构信息

Tufts University School of Medicine and Sackler School of Graduate Biomedical Sciences, Boston, Massachusetts 02111, USA.

出版信息

RNA. 2004 Apr;10(4):558-64. doi: 10.1261/rna.5238704.

DOI:10.1261/rna.5238704
PMID:15037764
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1370545/
Abstract

The eukaryotic poly(A) polymerase (PAP) is responsible for the posttranscriptional extension of mRNA 3' ends by the addition of a poly(A) tract. The recently published three-dimensional structures of yeast and bovine PAPs have made a more directed biochemical analysis of this enzyme possible. Based on these structures, the middle domain of PAP was predicted to interact with ATP. However, in this study, we show that mutations of conserved residues in this domain of yeast PAP, Pap1, do not affect interaction with ATP, but instead disrupt the interaction with RNA and affect the enzyme's ability to process substrate lacking 2' hydroxyls at the 3' end. These results are most consistent with a model in which the middle domain of PAP interacts directly with the recently extended RNA and pyrophosphate byproduct.

摘要

真核生物多聚腺苷酸聚合酶（PAP）负责通过添加多聚腺苷酸尾在转录后延伸mRNA的3'末端。最近发表的酵母和牛PAP的三维结构使得对该酶进行更有针对性的生化分析成为可能。基于这些结构，预测PAP的中间结构域与ATP相互作用。然而，在本研究中，我们表明酵母PAP（Pap1）该结构域中保守残基的突变并不影响与ATP的相互作用，而是破坏与RNA的相互作用，并影响该酶处理3'末端缺乏2'羟基底物的能力。这些结果与一个模型最为一致，在该模型中，PAP的中间结构域直接与最近延伸的RNA和焦磷酸副产物相互作用。