23S核糖体RNA中对核糖体亚基结合至关重要的碱基的定义。
Definition of bases in 23S rRNA essential for ribosomal subunit association.
作者信息
Maiväli Ulo, Remme Jaanus
机构信息
Department of Molecular Biology, Tartu University, Tartu, Estonia.
出版信息
RNA. 2004 Apr;10(4):600-4. doi: 10.1261/rna.5220504.
Abstract
The ribosome is a two-subunit molecular machine, sporting a working cycle that involves coordinated movements of the subunits. Recent structural studies of the 70S ribosome describe a rather large number of intersubunit contacts, some of which are dynamic during translocation. We set out to determine which intersubunit contacts are functionally indispensable for the association of ribosome subunits by using a modification interference approach. Modification of the N-1 position of A715, A1912, or A1918 in Escherichia coli 50S subunits is strongly detrimental to 70S ribosome formation. This result points to 23S rRNA helices 34 and 69, and thus bridges B2a and B4, as essential for ensuring stability of the 70S ribosome.
摘要
核糖体是一种由两个亚基组成的分子机器，其工作循环涉及亚基的协同运动。最近对70S核糖体的结构研究描述了相当多的亚基间接触，其中一些在转位过程中是动态的。我们着手通过使用修饰干扰方法来确定哪些亚基间接触对于核糖体亚基的结合在功能上是不可或缺的。对大肠杆菌50S亚基中A715、A1912或A1918的N-1位置进行修饰对70S核糖体的形成有强烈的不利影响。这一结果表明23S rRNA的螺旋34和69，也就是桥B2a和B4，对于确保70S核糖体的稳定性至关重要。
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