van den Boom R, Brama P A J, Kiers G H, DeGroot J, Barneveld A, van Weeren R R
Department of Equine Sciences, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 12, Utrecht, The Netherlands.
Equine Vet J. 2004 Mar;36(2):155-9. doi: 10.2746/0425164044868602.
Matrix metalloproteinases (MMPs) and tumour necrosis factor alpha (TNF-alpha) may be useful as biomarkers of joint disease or inflammation. However, activity of both MMPs and TNF-alpha in synovial fluid (SF) may be influenced by nonpathological factors such as arthrocentesis or exercise.
To investigate the influence of repeated arthrocentesis and exercise on MMP and TNF-alpha activities in SF from normal equine joints.
SF was collected from the left metacarpophalangeal, radiocarpal and tarsocrural joints of 16 horses. Eight of these horses were subsequently subjected to an exercise programme on a treadmill and 8 were box-rested as controls. Arthrocentesis was repeated 14, 145, 17 and 24 days after the start of the exercise programme. General MMP and TNF-alpha activities were determined in SF.
Repeated arthrocentesis caused a gradual increase but the exercise regimen no significant increase in MMP activity. There was a significant increase in TNF-alpha activity in SF collected from horses 2 h after cessation of the exercise programme.
When using MMPs as biomarkers for joint disease, at least 14 days should elapse after previous arthrocentesis before subsequent SF collection. Moderate exercise does not increase MMP activity in SF from normal joints and it may be possible to ignore this as a source of error in evaluating MMP activity in diseased joints.
基质金属蛋白酶（MMPs）和肿瘤坏死因子α（TNF-α）可能作为关节疾病或炎症的生物标志物。然而，滑液（SF）中MMPs和TNF-α的活性可能受到诸如关节穿刺或运动等非病理因素的影响。
研究重复关节穿刺和运动对正常马关节滑液中MMP和TNF-α活性的影响。
从16匹马的左掌指关节、桡腕关节和跗关节采集滑液。其中8匹马随后在跑步机上进行运动计划，8匹马圈养作为对照。在运动计划开始后14、145、17和24天重复进行关节穿刺。测定滑液中的总MMP和TNF-α活性。
重复关节穿刺导致MMP活性逐渐增加，但运动方案未使其显著增加。在运动计划停止2小时后采集的马的滑液中，TNF-α活性显著增加。
当使用MMPs作为关节疾病的生物标志物时，在先前关节穿刺后至少应间隔14天再采集后续滑液。适度运动不会增加正常关节滑液中的MMP活性，在评估患病关节的MMP活性时，可能可以忽略这一误差来源。
