Koruk Mehmet, Taysi Seyithan, Savas M Cemil, Yilmaz Omer, Akcay Fatih, Karakok Metin
Department of Gastroenterology, Gaziantep University Medical School, Gaziantep, Turkey.
Ann Clin Lab Sci. 2004 Winter;34(1):57-62.
Oxidative stress is an important pathophysiological mechanism in nonalcoholic steatohepatitis (NASH). To assess whether there are relationships between oxidative stress and antioxidant enzymes in the development of NASH, we investigated oxidative stress by measuring serum malondialdehyde (MDA) and nitric oxide (NO) and antioxidant status by measuring serum glutathione (GSH), glutathione peroxidase (GSH-Px), glutathione reductase (GR), and superoxide dismutase (SOD). The study included 18 patients (13 men, 5 women; mean age 42 yr) with biopsy proven NASH and 16 healthy volunteers (10 men, 6 women; mean age 38 yr). Serum levels of MDA, NO, GSH, GSH-Px, GR and SOD were determined by spectrophotometric methods. Serum levels (mean +/- SD) of MDA (6.7 +/- 1.6 vs 2.8 +/- 1.7 nmol/ml, p 0.0001), NO (135 +/- 28 vs 113 +/- 35 mmol/L, p 0.04), GSH (919 +/- 137 vs 770 +/- 128 mmol/L, p 0.003) were increased in patients with NASH vs controls. Serum levels of GSH-Px (1063 +/- 152 vs 1000 +/- 94 U/L) and GR (47 +/- 22 vs 40 +/- 21 U/L) were not singnificantly different in the patients vs controls. However, the serum level of SOD (1.24 +/- 0.32 vs 1.51 +/- 0.37 U/ml, p: 0.04) was significantly decreased. Impaired antioxidant defense mechanisms may be an important factor in the pathogenesis of NASH. Treatment approaches that affect the antioxidant enzymes may be beneficial in patients with NASH.
氧化应激是非酒精性脂肪性肝炎（NASH）重要的病理生理机制。为评估氧化应激与抗氧化酶在NASH发生发展过程中是否存在关联，我们通过检测血清丙二醛（MDA）和一氧化氮（NO）来研究氧化应激情况，并通过检测血清谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽还原酶（GR）和超氧化物歧化酶（SOD）来评估抗氧化状态。该研究纳入了18例经活检证实为NASH的患者（13例男性，5例女性；平均年龄42岁）以及16名健康志愿者（10例男性，6例女性；平均年龄38岁）。采用分光光度法测定血清中MDA、NO、GSH、GSH-Px、GR和SOD的水平。与对照组相比，NASH患者血清中MDA（6.7±1.6 vs 2.8±1.7 nmol/ml，p＜0.0001）、NO（135±28 vs 113±35 mmol/L，p＜0.04）、GSH（919±137 vs 770±128 mmol/L，p＜0.003）水平升高。患者与对照组血清中GSH-Px（1063±152 vs 1000±94 U/L）和GR（47±22 vs 40±21 U/L）水平无显著差异。然而，血清SOD水平（1.24±0.32 vs 1.51±0.37 U/ml，p：0.04）显著降低。抗氧化防御机制受损可能是NASH发病机制中的一个重要因素。影响抗氧化酶的治疗方法可能对NASH患者有益。
