红细胞膜空壳与完整红细胞作为光动力细胞研究中的互补模型。

Red blood cell ghosts and intact red blood cells as complementary models in photodynamic cell research.

作者信息

Kaestner Lars

机构信息

Institute for Molecular Cell Biology, Faculty of Medicine, Saarland University, Building 61, 66421 Homburg/Saar, Germany.

出版信息

Bioelectrochemistry. 2004 May;62(2):123-6. doi: 10.1016/j.bioelechem.2003.08.005.

DOI:10.1016/j.bioelechem.2003.08.005

PMID:15039014

Abstract

Recent research on erythrocytes as model cells for photodynamic therapy showed differing behaviour of certain photosensitisers in erythrocytes compared to other cells. Differences of dye accumulation in the cell membrane were proposed to be the reason for the distinct photodynamic effects. Using pheophorbide a as an example, the combination of erythrocyte ghosts as models to follow the dye accumulation in the cell membrane and intact erythrocytes as model cells to show the photodynamic damage is provided. Evidence for the correctness of the combination of erythrocyte ghosts and intact erythrocytes as a functioning model system in photodynamic cell research is provided using the confocal laser scanning microscopy on intact, pheophorbide a loaded erythrocytes.

摘要

近期将红细胞作为光动力疗法模型细胞的研究表明，与其他细胞相比，某些光敏剂在红细胞中的行为有所不同。细胞膜中染料积累的差异被认为是产生不同光动力效应的原因。以脱镁叶绿酸a为例，提供了红细胞血影作为模型来追踪细胞膜中染料积累，以及完整红细胞作为模型细胞来展示光动力损伤的组合。通过对完整的、负载脱镁叶绿酸a的红细胞进行共聚焦激光扫描显微镜检查，为红细胞血影和完整红细胞组合作为光动力细胞研究中一个有效模型系统的正确性提供了证据。