Coenye Tom, Vanlaere Elke, LiPuma John J, Vandamme Peter
Laboratorium voor Microbiologie, Universiteit Gent, KL Ledeganckstraat 35, B-9000 Gent, Belgium.
FEMS Immunol Med Microbiol. 2004 Apr 9;40(3):181-5. doi: 10.1016/S0928-8244(03)00307-9.
Stenotrophomonas maltophilia isolates have been recovered from various clinical samples, including the respiratory tract of cystic fibrosis (CF) patients, but this organism is also widespread in nature. Previously it has been shown that there is a considerable genomic diversity within S. maltophilia. The aims of our study were to determine the taxonomic resolution of restriction fragment length polymorphism (RFLP) analysis of the polymerase chain reaction-amplified gyrB gene for the genus Stenotrophomonas. Subsequently, we wanted to use this technique to screen a set of S. maltophilia isolates (with emphasis on a specific subset, isolates recovered from CF patients), to assess the genomic diversity within this group. In this study we investigated 191 Stenotrophomonas sp. isolates (including 40 isolates recovered from CF patients) by means of gyrB RFLP. The taxonomic resolution of gyrB RFLP, and hence its potential as an identification tool, was confirmed by comparison with results from published and novel DNA-DNA hybridisation experiments. Our data also indicate that the majority of CF isolates grouped in two clusters. This may indicate that isolates from specific genomic groups have an increased potential for colonisation of the respiratory tract of CF patients.
嗜麦芽窄食单胞菌分离株已从各种临床样本中分离得到，包括囊性纤维化（CF）患者的呼吸道，但这种微生物在自然界中也广泛存在。此前已表明，嗜麦芽窄食单胞菌内部存在相当大的基因组多样性。我们研究的目的是确定嗜麦芽窄食单胞菌属聚合酶链反应扩增的gyrB基因的限制性片段长度多态性（RFLP）分析的分类分辨率。随后，我们想用这项技术筛选一组嗜麦芽窄食单胞菌分离株（重点是从CF患者中分离得到的特定亚组），以评估该组内的基因组多样性。在本研究中，我们通过gyrB RFLP对191株嗜麦芽窄食单胞菌属分离株（包括从CF患者中分离得到的40株）进行了研究。通过与已发表的和新的DNA-DNA杂交实验结果进行比较，证实了gyrB RFLP的分类分辨率及其作为鉴定工具的潜力。我们的数据还表明，大多数CF分离株聚为两个簇。这可能表明来自特定基因组群的分离株在CF患者呼吸道定植的可能性增加。
