Ericsson Ulrika B, Andersson Martin E, Engvall Benita, Nordlund Pär, Hallberg B Martin
Department of Biochemistry and Biophysics, Stockholm University, Roslagstullsbacken 15, SE-114 21 Stockholm, Sweden.
Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):775-6. doi: 10.1107/S0907444904003506. Epub 2004 Mar 23.
Pseudouridine, the 5-ribosyl isomer of uridine, is the most common modification of structural RNA. The recently identified pseudouridine synthase TruD belongs to a widespread class of pseudouridine synthases without significant sequence homology to previously known families. TruD from Escherichia coli was overexpressed, purified and crystallized. The crystals diffract to a minimum Bragg spacing of 2.4 A and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 63.4, b = 108.6, c = 111.7 A.
假尿苷是尿苷的5-核糖基异构体，是结构RNA中最常见的修饰形式。最近鉴定出的假尿苷合酶TruD属于一类广泛存在的假尿苷合酶，与先前已知的家族没有明显的序列同源性。来自大肠杆菌的TruD被过量表达、纯化并结晶。晶体的衍射最小布拉格间距为2.4 Å，属于空间群P2(1)2(1)2(1)，晶胞参数a = 63.4、b = 108.6、c = 111.7 Å。
