Chung Shu-Ying, Lin Jing-Ying, Lin Ming-Cheng, Liu Hui-Ming, Wang Ming-Fu, Cheng Fu-Chou
Department of Food and Nutrition, Providence University, Taichung 433, Taiwan, ROC.
Med Sci Monit. 2004 Apr;10(4):BR105-8.
In this study, we administered intraperitoneal magnesium sulfate (90 mg/kg), either alone or in combination with FK506 (0.5 mg/kg) in order to investigate the neuroprotective synergetic effects of both, 30 min before permanent occlusion of the middle cerebral artery (MCA) in gerbil.
MATERIAL/METHODS: Postmortem infarct volume was determined by quantitative image analysis of triphenyltetrazolium-stained brain sections, collected at 1 h, 3 h and 24 h after initiation of cerebral ischemia, to measure consistent infarct volumes of the post -ischemic gerbil brain. Twenty-four male gerbils were intraperitoneally injected with MgSO4 (n=7), FK506 (n=8), or MgSO4 +FK506 (n=9), 30 min before permanent MCA occlusion. The brains of the treated groups were stained at 24 h after initiation of cerebral ischemia.
Significant and consistent infarct volumes (78.3 +/- 3.6 mm3) were observed in the 24 H control group. The infarct volume in the MgSO4 + FK506 group was significantly reduced at each slice, compared to the untreated 24 H control group (P<0.05). Magnesium sulfate reduced total infarct volume by 25.4% (P<0.05), FK506 by 41% (P<0.05), and MgSO4 +FK506 by 50.5% (P<0.05). Also, the infarct volume (38.7 +/- 5.2 mm3) in the MgSO4 + FK506 group was the smallest in all the treated groups, near the volumes seen in the untreated 3H group.
Acute administration of a combination of MgSO4 and FK506 has a synergetic efficacy against brain damage by permanent focal cerebral ischemia.
在本研究中，我们在沙土鼠大脑中动脉（MCA）永久性闭塞前30分钟腹腔注射硫酸镁（90毫克/千克），单独使用或与FK506（0.5毫克/千克）联合使用，以研究两者的神经保护协同作用。
材料/方法：通过对在脑缺血开始后1小时、3小时和24小时收集的经三苯基四氮唑染色的脑切片进行定量图像分析，确定死后梗死体积，以测量缺血后沙土鼠脑的一致梗死体积。24只雄性沙土鼠在MCA永久性闭塞前30分钟腹腔注射硫酸镁（n = 7）、FK506（n = 8）或硫酸镁+ FK506（n = 9）。治疗组的大脑在脑缺血开始后24小时进行染色。
在24小时对照组中观察到显著且一致的梗死体积（78.3±3.6立方毫米）。与未治疗的24小时对照组相比，硫酸镁+ FK506组在每个切片中的梗死体积均显著减小（P < 0.05）。硫酸镁使总梗死体积减少25.4%（P < 0.05），FK506减少41%（P < 0.05），硫酸镁+ FK506减少50.5%（P < 0.05）。此外，硫酸镁+ FK506组的梗死体积（38.7±5.2立方毫米）在所有治疗组中最小，接近未治疗的3小时组的体积。
急性联合给予硫酸镁和FK506对永久性局灶性脑缺血所致脑损伤具有协同疗效。
