Mantione Kirk J, Stefano George B
Neuroscience Research Institute, State University of New York College at Old Westbury, Old Westbury, NY 11568, USA.
Med Sci Monit. 2004 Apr;10(4):MT47-9.
Amperometric nitric oxide probes are critical in evaluating real-time nitric oxide levels. This valuable tool enables one to measure spontaneous baseline levels of nitric oxide as well as 'puffs' of the gaseous signal molecule that may last for only seconds to minutes. However, in the past, many probes suffered from a lack of sensitivity, durability and reliability, causing investigators to design numerous controls to support their data.
MATERIAL/METHODS: Our laboratory evaluated the new ISO-NOPF100 NO probe manufactured by World Precision Instruments of Sarasota, Florida. An invertebrate in vivo heart preparation was used, which presents a high degree of difficuly in obtaining nitric oxide measurements due to space limitations, resulting in physical contact of the probe with tissues. Additionally, we used in vitro invertebrate ganglionic preparations as a comparison since this tissue releases spontaneous and low levels of NO.
Calibration of the new probe demonstrated high linearity and sensitivity. The detection limit for this new probe was determined to be approximately two times lower than probes previously used in our laboratory. Basal nitric oxide fluctuations in Mytilus edulis heart and excised ganglia were able to be resolved in the sub-nanomolar range.
The ISO-NOPF100 NO probe represents a significant advancement for measuring nitric oxide in real-time.
电流型一氧化氮探头对于评估实时一氧化氮水平至关重要。这一有价值的工具能够测量一氧化氮的自发基线水平以及气态信号分子的“脉冲”，这些“脉冲”可能仅持续数秒至数分钟。然而，过去许多探头存在灵敏度、耐用性和可靠性不足的问题，导致研究人员设计大量对照来支持他们的数据。
材料/方法：我们实验室评估了由佛罗里达州萨拉索塔市世界精密仪器公司生产的新型ISO-NOPF100一氧化氮探头。使用了一种无脊椎动物体内心脏标本，由于空间限制，在获取一氧化氮测量值时存在高度困难，这导致探头与组织发生物理接触。此外，我们使用体外无脊椎动物神经节标本作为对照，因为这种组织会自发释放低水平的一氧化氮。
新探头的校准显示出高线性和灵敏度。该新探头的检测限被确定为比我们实验室之前使用的探头低约两倍。在贻贝心脏和离体神经节中，基础一氧化氮波动能够在亚纳摩尔范围内得到解析。
ISO-NOPF100一氧化氮探头代表了实时测量一氧化氮方面的重大进展。
