Morito T, Nakamura T, Nakai H, Tanimoto K, Horiuchi Y, Juji T
Int Arch Allergy Appl Immunol. 1978;57(6):521-8. doi: 10.1159/000232147.
The effects of aggregated human IgG, human anti-Ia-like antibody, and anti-beta2-microglobulin on mixed lymphocyte culture (MLC) were studied. Aggregated human IgG inhibited both stimulatory and responding activities in MLC. When Fc-receptor-bearing cells were removed from responding cells, the inhibitory activity of aggregated IgG was markedly reduced. The result suggested that the inhibition of MLC by aggregated IgG is primarily based on the blocking of Fc-receptor-bearing cells contained in the stimulator cells. In addition, the removal of Fc-receptor-bearing cells from stimulatory cells resulted in the loss of MLC response. Anti-Ia-like antibodies contained in anti-HLA sera and B-cell-specific human alloantisera also inhibited stimulatory activities in MLC. Rabbit antiserum against human beta2-microglobulin showed inhibitions of both stimulatory and responding activities. These results suggested the close association of human MLC stimulator site with Fc receptor and Ia-like antigen and also some relation of beta2-microglobulin with MLC reaction.
研究了聚集的人IgG、人抗Ia样抗体和抗β2微球蛋白对混合淋巴细胞培养（MLC）的影响。聚集的人IgG抑制MLC中的刺激活性和反应活性。当从反应细胞中去除携带Fc受体的细胞时，聚集IgG的抑制活性明显降低。结果表明，聚集IgG对MLC的抑制主要基于阻断刺激细胞中所含的携带Fc受体的细胞。此外，从刺激细胞中去除携带Fc受体的细胞导致MLC反应丧失。抗HLA血清和B细胞特异性人同种抗血清中所含的抗Ia样抗体也抑制MLC中的刺激活性。兔抗人β2微球蛋白血清显示出对刺激活性和反应活性的抑制。这些结果表明人MLC刺激位点与Fc受体和Ia样抗原密切相关，并且β2微球蛋白与MLC反应也存在某种关系。
