Nielsen H E, Heron I, Koch C
Transplantation. 1977 Jan;23(1):73-7. doi: 10.1097/00007890-197701000-00013.
We have examined the effect in mixed leucocyte culture (MLC) with rat cells of the IgG fraction of alloantibodies against the major histocompatibility complex (anti-BN major) and against some of the minor histocompatibility antigens. Anti-BN major specifically inhibited the MLC response, both when BN cells were responding and stimulating cells. Absorption with BN erythrocytes completely removed the haemagglutinating antibodies but had no effect of MLC inhibition, while absorption with BN lymphocytes removed MLC inhibition as well as haemagglutinating antibodies. The F(ab')2 fragments of anti-BN major did not inhibit MLC, although they still had the capacity to combine specifically with BN lymphocytes. We therefore suggest that the inhibition of MLC responses by alloantibodies is either because of a cytotoxic effect (even if cytotoxicity could not be demonstrated in unmixed cultures) or a blocking of antibody-coated lymphocytes by Fc receptor-bearing cells. Antibodies against minor histocompatibility antigens did not inhibit MLC.
我们研究了抗主要组织相容性复合体（抗-BN主要）和一些次要组织相容性抗原的同种抗体IgG组分在大鼠细胞混合白细胞培养（MLC）中的作用。抗-BN主要特异性抑制MLC反应，无论是BN细胞作为反应细胞还是刺激细胞时。用BN红细胞吸收可完全去除血凝抗体，但对MLC抑制无影响，而用BN淋巴细胞吸收则可去除MLC抑制以及血凝抗体。抗-BN主要的F(ab')2片段虽仍具有与BN淋巴细胞特异性结合的能力，但不抑制MLC。因此，我们认为同种抗体对MLC反应的抑制要么是由于细胞毒性作用（即使在未混合培养中未证明细胞毒性），要么是由于带有Fc受体的细胞对抗体包被的淋巴细胞的封闭作用。抗次要组织相容性抗原的抗体不抑制MLC。
