Schoske Richard, Vallone Peter M, Kline Margaret C, Redman Janette W, Butler John M
Biotechnology Division, National Institute of Technology, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899, USA.
Forensic Sci Int. 2004 Jan 28;139(2-3):107-21. doi: 10.1016/j.forsciint.2003.10.007.
Two Y-chromosome short tandem repeat (STR) multiplex polymerase chain reaction (PCR) assays were used to generate haplotypes for 19 single copy and 3 multi-copy Y-STRs. A total of 27 PCR products were examined in each sample using the following loci: DYS19, DYS385 a/b, DYS388, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS426, DYS437, DYS438, DYS439, DYS447, DYS448, DYS450, DYS456, DYS458, DYS460, DYS464 a/b/c/d, H4, and YCAII a/b. The first multiplex is the Y-STR 20plex previously described by Butler et al. [Forensic Sci. Int. 129 (2002) 10]. The second multiplex is a novel Y-STR 11plex and includes DYS385 a/b, DYS447, DYS448 and the new markers DYS450, DYS456, DYS458, and DYS464 a/b/c/d. These two multiplexes were tested on 647 males from three United States population sample sets: 260 African Americans, 244 Caucasians, and 143 Hispanics. Haplotype comparisons between common loci included in the 20plex and 11plex assays as well as commercially available kits found excellent agreement across a sampling of the population samples. The multi-copy loci DYS464, DYS385, and YCAII were the most polymorphic followed by the following single copy Y-STRs: DYS458, DYS390, DYS447, DYS389II, DYS448, and DYS456. Samples containing the most common type in the European database could be well resolved with additional markers beyond the minimal haplotype loci.
采用两种Y染色体短串联重复序列（STR）复合聚合酶链反应（PCR）分析方法，对19个单拷贝和3个多拷贝Y-STR生成单倍型。在每个样本中，使用以下基因座检测总共27个PCR产物：DYS19、DYS385 a/b、DYS388、DYS389I/II、DYS390、DYS391、DYS392、DYS393、DYS426、DYS437、DYS438、DYS439、DYS447、DYS448、DYS450、DYS456、DYS458、DYS460、DYS464 a/b/c/d、H4以及YCAII a/b。第一个复合体系是Butler等人之前描述的Y-STR 20复合体系[《法医学国际杂志》129（2002年）10]。第二个复合体系是一个新的Y-STR 11复合体系，包括DYS385 a/b、DYS447、DYS448以及新标记DYS450、DYS456、DYS458和DYS464 a/b/c/d。对来自美国三个群体样本集的647名男性进行了这两种复合体系的检测：260名非裔美国人、244名白种人以及143名西班牙裔。对20复合体系和11复合体系以及市售试剂盒中包含的共同基因座之间的单倍型比较发现，在对群体样本的抽样中一致性极佳。多拷贝基因座DYS464、DYS385和YCAII多态性最高，其次是以下单拷贝Y-STR：DYS458、DYS390、DYS447、DYS389II、DYS448和DYS456。欧洲数据库中包含最常见类型的样本，通过最小单倍型基因座之外的其他标记可以很好地分辨。
